食品科学 ›› 2018, Vol. 39 ›› Issue (4): 290-295.doi: 10.7506/spkx1002-6630-201804043

• 安全检测 • 上一篇    下一篇

软饮料中人工合成色素快速测定试纸条的研制

吴红静1,2,王维亚3,叶维佳2,杨普宁2,黄志兵1,涂追1,*   

  1. (1.南昌大学 食品科学与技术国家重点实验室,江西南昌 330047;2.南昌大学科学技术学院,江西南昌 330029;3.南昌市食品药品检验所,江西南昌 330038)
  • 出版日期:2018-02-25 发布日期:2018-02-02
  • 基金资助:
    国家自然科学基金青年科学基金项目(31301479);江西省教育厅科学技术研究项目(GJJ151502); 南昌大学科学技术学院自然科学基金项目(2013-ZR-05)

Shake-and-Read Test Strips for Discriminating Synthetic Food Colorants from Natural Colorants in Soft Drinks

WU Hongjing1,2, WANG Weiya3, YE Weijia2, YANG Puning2, HUANG Zhibing1, TU Zhui1,*   

  1. (1. State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China; 2. College of Science and Technology, Nanchang University, Nanchang 330029, China; 3. Nanchang Institute for Food and Drug Control, Nanchang 330038, China)
  • Online:2018-02-25 Published:2018-02-02

摘要: 基于聚酰胺薄膜与人工合成色素之间的特异性吸附的原理,建立一种快速、低成本的测定软饮料中最常用的3种人工合成色素(胭脂红E124、柠檬黄E102、日落黄E110)的方法。在优化后的检测条件下,3种色素的最低检测限分别为0.4、0.3mg/L和0.4mg/L,检测时间10min。对14个市售样品进行检测,结果表明,建立的快速检测方法与薄层色谱和高效液相色谱两种方法的结果吻合。该方法操作简单、不需要仪器设备,凭肉眼判读,可用于现场检测和快速筛查,为控制和监测合成色素的滥用或过量添加提供技术途径。

关键词: 人工合成色素, 聚酰胺, 快速测定, 软饮料

Abstract: A test strip method was used to detect three synthetic colorants commonly used in soft drinks. Under the optimal conditions, the entire detection procedure was completed within 10 min, and the limits of detection (LODs) of three synthetic food colorants (SFCs), namely, Ponceau 4R (E124), Tartrazine (E102), and Sunset Yellow (E110), were 0.4, 0.3, and 0.4 mg/L, respectively. The proposed method was compared with thin-layer chromatography and high-performance liquid chromatography. The results of validation with 14 commercial samples indicated the reliability and sensitivity of the strip test method to the presence of the three SFCs in soft drinks. This study indicated that the polyamide film-based test strip exhibited the potential to monitor illegal or improper addition of SFCs.

Key words: synthetic colorants, polyamide, rapid detection, soft drink

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