磺胺甲噁唑和磺胺嘧啶在青石斑鱼组织中的分布及代谢规律

doi:10.7506/spkx1002-6630-201804045

食品科学 ›› 2018, Vol. 39 ›› Issue (4): 301-306.doi: 10.7506/spkx1002-6630-201804045

磺胺甲噁唑和磺胺嘧啶在青石斑鱼组织中的分布及代谢规律

邓建朝1，丁军伟1,2，杨贤庆1,*，李来好1，胡晓1，吴燕燕1，陈胜军1，杨少玲1，赵庆志1,2

（1.中国水产科学研究院南海水产研究所，农业部水产品加工重点实验室，广东省渔业生态环境重点实验室，广东广州 510300；2.上海海洋大学食品学院，上海 201306）

出版日期:2018-02-25 发布日期:2018-02-02
基金资助:
“十二五”国家科技支撑计划项目（2015BAK36B06；2015BAD17B03）；国家农产品质量安全风险评估项目（GJFP201501201；GJFP201700901）；广东省海洋渔业科技与产业发展专项（A201501C02）；现代农业产业技术体系专项（CARS-47）

Distribution and Metabolism Patterns of Sulfamethoxazole and Sulfadiazine in Yellow Grouper (Epinephelus awoara) Tissues

DENG Jianchao1, DING Junwei1,2, YANG Xianqing1,*, LI Laihao1, HU Xiao1, WU Yanyan1, CHEN Shengjun1, YANG Shaoling1, ZHAO Qingzhi1,2

(1. Key Laboratory of Aquatic Product Processing, Ministry of Agriculture, Guangdong Provincial Key Laboratory of Fishery Ecology and Environment, South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510300, China;2. College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, China)

Online:2018-02-25 Published:2018-02-02

摘要/Abstract

摘要： 通过一次性投喂各含有200 mg/kg磺胺甲噁唑（sulfamethoxazole，SMZ）和磺胺嘧啶（sulfadiazine，SDZ）的饲料，研究两种药物在青石斑鱼中各组织分布与消除规律。采用超高效液相色谱-串联质谱检测青石斑鱼各组织中SMZ和SDZ的含量，并用内标法定量。结果表明，SMZ在青石斑鱼各组织中的最大含量：肝脏、背肌、血浆、肾脏和鳃依次为827.97μg/kg、776.70μg/kg、610.29μg/L、432.14μg/kg和345.18μg/kg。SDZ在青石斑鱼各组织中的最大含量：肝脏、背肌、鳃、血浆和肾脏依次为895.30μg/kg、660.55μg/kg、431.88μg/kg、419.56μg/L和310.67μg/kg。SMZ在青石斑鱼各组织中的半衰期：肾脏、鳃、背肌、血浆和肝脏半衰期依次为26.65、21.00、20.38、18.73h和16.90h。SDZ在青石斑鱼各组织中的半衰期：肾脏、血浆、鳃、背肌和肝脏依次为31.50、27.72、24.75、21.66h和18.24h。SMZ和SDZ在青石斑鱼肝脏中半衰期最短，代谢速度最快；在肾脏中半衰期最长，代谢速度最慢。在水温（25±2）℃条件下，SMZ和SDZ各200mg/kg的剂量同时单次投喂青石斑鱼，建议休药期不低于3d。SMZ和SDZ代谢规律研究为磺胺类药物在水产品中的合理使用提供了参考。

关键词: 青石斑鱼, 磺胺甲噁唑, 磺胺嘧啶, 超高效液相色谱-串联质谱, 代谢规律

Abstract: In this study, yellow grouper (Epinephelus awoara) were administered with a single dose (200 mg/kg) of sulfamethoxazole (SMZ) and sulfadiazine (SDZ) incorporated in the diet to investigate the distribution and elimination patterns of SMZ and SDZ in various fish tissues. Both sulfonamides were detected by high performance liquid chramotography-tandem mass spectrometry (HPLC-MS/MS) and quantified by the internal standard method. The results showed that SMZ concentrations in fish tissues were in the following order: liver 827.97 (μg/kg) > muscle (776.70 μg/kg) > blood (610.29 μg/L) > kidney (432.14 μg/kg) > gill (345.18 μg/kg), while SDZ concentrations in fish tissues were in the following order: liver (895.30 μg/kg) > muscle (660.55 μg/kg) > gill (431.88 μg/kg) > blood (419.56 μg/L) > kidney (310.67 μg/kg). The elimination half-life of SMZ in fish tissues was in the following order: kidney (26.65 h) > gill (21.00 h) > muscle (20.38 h) > blood (18.73 h) > liver (16.90 h), while that of SDZ in fish tissues was in the following order: kidney (31.50 h) > blood (27.72 h) > gill (24.75 h) > muscle (21.66 h) > liver (18.24 h). The elimination half-lives of two sulfonamides in liver were much shorter than all other tissues and they were metabolized at a faster rate in liver. On the contrary, the sulfonamides showed the longest elimination half-life and were metabolized at the fastest rate in kidney among the tissues tested. After simultaneous administration of single-dose SMZ and SDZ to fish living at (25 ± 2) ℃, the withdrawal period should last at least 3 days. In conclusion, our study provides a theoretical and experimental basis for reasonable application of SMZ and SDZ in fish farming.

Key words: yellow grouper (Epinephelus awoara), sulfamethoxazole, sulfadiazine, HPLC-MS/MS, metabolic patterns

中图分类号:

S948

邓建朝，丁军伟，杨贤庆，李来好，胡晓，吴燕燕，陈胜军，杨少玲，赵庆志. 磺胺甲噁唑和磺胺嘧啶在青石斑鱼组织中的分布及代谢规律[J]. 食品科学, 2018, 39(4): 301-306.

DENG Jianchao, DING Junwei, YANG Xianqing, LI Laihao, HU Xiao, WU Yanyan, CHEN Shengjun, YANG Shaoling, ZHAO Qingzhi. Distribution and Metabolism Patterns of Sulfamethoxazole and Sulfadiazine in Yellow Grouper (Epinephelus awoara) Tissues[J]. FOOD SCIENCE, 2018, 39(4): 301-306.

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磺胺甲噁唑和磺胺嘧啶在青石斑鱼组织中的分布及代谢规律

Distribution and Metabolism Patterns of Sulfamethoxazole and Sulfadiazine in Yellow Grouper (Epinephelus awoara) Tissues

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