关键词: SSR指纹图谱, 绿豆, 品种鉴定

Abstract: Objective: This study aimed to establish a DNA fingerprint for rapid and accurate identification of mung bean varieties grown in Liaoning. Methods: A total of 48 mung bean samples from different varieties were used to construct a fingerprint database and a DNA fingerprint with 15 pairs of polymorphic simple sequence repeat (SSR) primers. Results: A total of 58 alleles were detected, 2–7 of which, with an average of 3.9, were detected with each pair of the primers used, and 13 specific bands were obtained, accounting for about 22.4% of the total. The polymorphic information content (PIC) varied from 0.114 5–0.776 4, with an average of 0.378 4. Five pairs of efficient primers were selected. The discrimination rate obtained with their combination was as high as 75%, while a discrimination rate of 89.68% was obtained with a combination of all 15 primer pairs. Based on these results, the DNA fingerprint database composed of the digits 0 and 1 and the SSR fingerprint were established, which allowed unequivocal?identification of all the varieties except a few unique ones. Conclusion: The SSR fingerprint presented in this study can open up a new way of identifying mung bean varieties and provide a basis for mung resource evaluation and protection as well as breeding of new varieties.

Key words: SSR fingerprinting, mung bean, variety identification