食品科学 ›› 2011, Vol. 32 ›› Issue (11): 25-28.doi: 10.7506/spkx1002-6630-201111006

• 基础研究 • 上一篇    下一篇

扁枝槲寄生多糖体外抗氧化活性

钟文武,彭文书,余正云,陈毅坚   

  1. 1.云南民族大学化学与生物技术学院 2.西双版纳职业技术学院
  • 出版日期:2011-06-15 发布日期:2011-05-13
  • 基金资助:
    云南省教育厅科学研究基金项目(2010Y428)

in vitro Antioxidant Activity of Polysaccharides from Viscum articulatum

ZHONG Wen-wu1,PENG Wen-shu1,YU Zheng-yun2,CHEN Yi-jian1,*   

  1. 1. School of Chemistry and Biotechnology,Yunnan University of Nationalities, Kunming 650500, China; 2. Xishuangbanna Vocational and Technical Institute, Xishuangbanna 666100, China
  • Online:2011-06-15 Published:2011-05-13

摘要: 采用水提醇沉的方法对两种不同寄主的扁枝槲寄生中多糖进行提取,测定其多糖的含量,并利用分光光度法研究两种样品中的粗多糖对Fenton反应产生的羟自由基、邻苯三酚自氧化产生的超氧阴离子自由基以及DPPH自由基的清除作用,同时利用硫代巴比妥酸(TBA)分光光度法研究两种样品粗多糖对羟自由基诱发卵磷脂脂质过氧化损伤的抑制作用。结果表明:两种样品粗多糖对自由基有一定的清除作用,对卵磷脂脂质过氧化损伤也有一定的抑制作用,但随着寄主的不同,两种样品粗多糖的活性有所差异。

关键词: 扁枝槲寄生, 粗多糖, 抗氧化活性

Abstract: Crude polysaccharide samples respectively obtained from Viscum articulatum from two different hosts by microwave-assisted aqueous extraction and subsequent ethanol precipitation were determined for their polysaccharide contents. The abilities of the two polysaccharide samples to scavenge hydroxyl free radicals generated by Fenton reaction, superoixde anion free radicals generated by autoxidation of 1,2,3-benzenetriol and DPPH radicals were spectrometrically evaluated. Meanwhile, their suppressing effects on hydroxyl radical-induced lipid peroxidation of lecithin were spectrometrically examined by thiobarbituric acid method. The results showed that both crude polysaccharides had obvious scavenging effect on active radicals and inhibitory effect on lecithin peroxidation to some extent. However, these antioxidant effects differed between the two polysaccharide samples.

Key words: Viscum articulatum, crude polysaccharide, antioxidation

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