食品科学 ›› 2011, Vol. 32 ›› Issue (12): 54-56.doi: 10.7506/spkx1002-6630-201112012

• 工艺技术 • 上一篇    下一篇

养殖大黄鱼片脱脂工艺优化

徐海菊   

  1. 台州科技职业学院
  • 出版日期:2011-06-25 发布日期:2011-06-10
  • 基金资助:
    台州市科技计划项目(091TG03)

Optimization of Degreasing of Cultured Large Yellow Croaker (Pseudosciaena crocea) Fillets with Alkaline Lipase

XU Hai-ju   

  1. Taizhou Vocational College of Science and Technology, Taizhou 318020, China
  • Online:2011-06-25 Published:2011-06-10

摘要: 选用碱性脂肪酶对大黄鱼鱼片酶解脱脂工艺进行优化。以脱脂率为考核指标,采用单因素试验与正交试验相结合的方法,对大黄鱼鱼片进行脱脂。结果表明:采用碱性脂肪酶对大黄鱼脱脂的最适工艺条件为温度32℃、反应pH8.5、酶用量60U/mL、酶解时间60min、鱼与酶解液质量比1:4。在最适酶解条件下,大黄鱼鱼片的脱脂率为67.32%。

关键词: 养殖大黄鱼, 碱性脂肪酶, 脱脂率

Abstract: In order to achieve maximum degreasing rate, the hydrolysis of cultured large yellow croaker fillets by alkaline lipase was optimized by the combined use of single factor and orthogonal array design methods. The optimum hydrolysis conditions for degreasing large yellow croaker fillets were temperature 32 ℃, reaction pH 8.5, enzyme concentration 60 U/mL, reaction time 60 min, substrate-to-enzyme solution ratio 1:4 (by mass), respectively. Under these conditions, the degreasing rate was 67.32%.

Key words: cultured large yellow croaker (Pseudosciaena crocea), alkaline lipase, degreasing rate

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