食品科学 ›› 2011, Vol. 32 ›› Issue (12): 83-87.doi: 10.7506/spkx1002-6630-201112019

• 工艺技术 • 上一篇    下一篇

石蒜总生物碱的提取及其对酒精发酵过程的抑制机理

杨 馨,张 梁,丁重阳,石贵阳*   

  1. 工业生物技术教育部重点实验室,江南大学生物工程学院生物资源与生物能源研究中心
  • 出版日期:2011-06-25 发布日期:2011-06-10
  • 基金资助:
    2008 年度江苏省“青蓝工程”科技创新团队资助项目

Extraction of Total Alkaloids from Lycoris radiata Bulbs and Its Inhibitory Effect and Mechanism against Alcohol Fermentation by Saccharomyces cereviviae

YANG Xin,ZHANG Liang,DING Zhong-yang,SHI Gui-yang*   

  1. Key Laboratory of Industrial Biotechnology, Ministry of Education, Center for Bioresources and Bioenergy, School of Biotechnology, Jiangnan University, Wuxi 214122, China
  • Online:2011-06-25 Published:2011-06-10

摘要: 基于对石蒜生物碱抑菌作用的研究,结合生物碱的药用价值,提出酒精发酵前提取生物碱的必要性。通过单因素试验探讨生物碱的提取方式和提取条件对生物碱提取率的影响,结果表明,提取剂为甲醇、料醇比1:15(g/mL)、提取温度60℃、提取时间1h、提取液pH11.0条件下,生物碱的提取量达28.70mg/g;进一步对含有生物碱和不含生物碱的两种石蒜原料进行酒精发酵过程比较,结果显示,石蒜生物碱对糖化酶活性和工业酒精酵母的生长均具有明显抑制作用,对α-淀粉酶则影响较小,初步揭示了石蒜生物碱对酒精发酵过程的抑制机理。

关键词: 石蒜, 生物碱提取, 酒精发酵, 抑制机理

Abstract: The anti-Saccharomyces cereviviae effect and notable medicinal value of Lycoris radiata alkaloids highlight the importance and necessity of total alkaloids extraction before ethanol fermentation from L. radiata bulbs. In the present study, one-factor-at-at-time experiments were conducted to explore the extraction efficiency of total alkaloids from L. radiata bulbs with respect to five operating parameters including extraction solvent type, temperature, extraction time, solid-to-liquid ratio and pH. The results showed that the extraction efficiency of total alkaloids was up to 28.70 mg/g under the following conditions: methanol as extraction solvent, solid-to-liquid ratio of 1:15 (g/mL), temperature of 60 ℃, pH 11.0 and extraction time of 1 h. Further, the fermentation performance of L. radiata bulbs for producing ethanol was compared before and after the removal of total alkaloids. As a result, the presence of total alkaloids had dramatic inhibitory effect on glucoamylase activity and the growth of S. cereviviae and little effect on α-amylase activity. This can preliminarily explain the inhibitory mechanism of Lycoris radiata alkaloids on ethanol fermentation.

Key words: Lycoris radiata, alkaloids extraction, ethanol fermentation, inhibitory mechanism

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