麻叶千里光中酚类化合物提取及测定

doi:10.7506/spkx1002-6630-201112021

食品科学 ›› 2011, Vol. 32 ›› Issue (12): 92-95.doi: 10.7506/spkx1002-6630-201112021

麻叶千里光中酚类化合物提取及测定

高淑云

徐州工程学院化学化工学院

出版日期:2011-06-25 发布日期:2011-06-10
基金资助:
江苏省高校自然科学基础研究项目(07KJD550203)；江苏省大学生实践创新训练计划项目(2008002)

Extraction and Analysis of Phenolic Compounds in Senecio cannabifolius

GAO Shu-yun

College of Chemistry and Chemical Engineering, Xuzhou Institute of Technology, Xuzhou 221008, China

Online:2011-06-25 Published:2011-06-10

摘要/Abstract

摘要： 目的：以麻叶千里光为原料，采用微波-超声波协同提取法提取麻叶千里光中酚类化合物并测定其含量。方法：通过对微波-超声波协同提取时间、提取功率及提取剂体积分数进行单因素及正交试验，确定以乙醇为提取剂，提取麻叶千里光中酚类化合物的最佳工艺条件；然后采用紫外光谱法和薄层扫描法测定取麻叶千里光提取液中酚类化合物含量。结果：最佳提取工艺为微波提取时间120s、微波功率90W、乙醇体积分数75%。紫外光谱法测定麻叶千里光提取液中酚类化合物回收率92.30%，精密度2.204%，总酚含量0.1276%；薄层扫描法测定麻叶千里光提取液中酚类化合物回收率98.52%，精密度1.011%，总酚含量0.2044%。结论：采用紫外光谱法和薄层扫描法测定麻叶千里光中酚类化合物，其两种方法均可行，薄层扫描法测定麻叶千里光中酚类化合物比较准确。

关键词: 麻叶千里光, 提取, 多酚, 薄层扫描法, 紫外光谱法

Abstract: Objective: To optimize conditions for simultaneous microwave-ultrasonic assisted extraction of phenolic compounds from Senecio cannabifolius using ethanol as extraction solvent and to determine total phenol content in Senecio cannabifolius based on optimized microwave-ultrasonic assisted extraction process. Methods: Process conditions optimization was performed using one-factor-at-a-time and orthogonal array design methods. The extraction yield of phenolic compounds was investigated with respect to three process parameters such as microwave power, microwave treatment time and ethanol concentration. Determination of total phenol content was performed respectively using ultraviolet spectrophotometry and thin layer chromatography (TLC) scanning method. Results: The optimal extraction conditions were microwave treatment time of 120 s, microwave power of 90 W and ethanol concentration of 75%. In the ultraviolet spectrophotometeric determination, a mean spike recovery rate of 92.30%, a precision of 2.204% and a total phenol content of 0.1276% were observed, and those obtained by TLC scanning determination were 98.52%, 1.011% and 0.2044%, respectively. Conclusion: Both analytical methods above are applicable for the determination of phenolic compounds, and TLC scanning is a more accurate method.

Key words: Senecio cannabifolius, extraction, polyphenols, thin layer chromatography(TLC) scanning, ultraviolet spectrophotometry

中图分类号:

R284.2

高淑云. 麻叶千里光中酚类化合物提取及测定[J]. 食品科学, 2011, 32(12): 92-95.

GAO Shu-yun. Extraction and Analysis of Phenolic Compounds in Senecio cannabifolius[J]. FOOD SCIENCE, 2011, 32(12): 92-95.

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麻叶千里光中酚类化合物提取及测定

Extraction and Analysis of Phenolic Compounds in Senecio cannabifolius

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