食品科学 ›› 2011, Vol. 32 ›› Issue (12): 96-100.doi: 10.7506/spkx1002-6630-201112022

• 工艺技术 • 上一篇    下一篇

赤芍中芍药苷、芍药内酯苷双指标考察赤芍总苷提纯工艺优化

陈立江段洪云张 胜,朱鹏飞,刘 宇,王程程   

  1. 1.辽宁大学药学院 2.辽宁省药物研究院 3.首都医科大学中医药学院
  • 出版日期:2011-06-25 发布日期:2011-06-10
  • 基金资助:
    辽宁省自然科学基金项目(20092015);沈阳市科学技术计划项目(F10-205-1-28); 辽宁大学“211 工程”三期建设项目

Process Optimization for Simultaneous Extraction and Purification of Paeoniflorin and Albiflorin from Radix Paeoniae Rubra

CHEN Li-jiang1,DUAN Hong-yun1,ZHANG Sheng2,ZHU Peng-fei3,LIU Yu1,WANG Cheng-cheng1   

  1. 1. School of Pharmacy, Liaoning University, Shenyang 110036, China;2. Liaoning Institute of Medicine, Shenyang 110015, China;3. School of Chinese Medicine, Capital Medical University, Beijing 100069, China
  • Online:2011-06-25 Published:2011-06-10

摘要: 目的:双指标考察赤芍的提取纯化工艺。方法:以芍药苷和芍药内酯苷为指标,采用正交试验设计,确定了赤芍的最佳提取工艺,采用大孔吸附树脂纯化赤芍提取物。结果:赤芍的最佳提取工艺为:70%乙醇用量为8mL/g、回流提取3次,每次2h。最佳纯化工艺为:选用AB-8型大孔吸附树脂,径高比为1:8,最大上样量为4.4BV,水洗量为3BV,洗脱流速为1.5BV/h,洗脱剂为40%乙醇,洗脱剂用量为6BV。结论:优选出的提取纯化工艺简便、实用,得到的赤芍总苷纯度高。

关键词: 赤芍, 提取, 纯化, 大孔吸附树脂

Abstract: Objective: To optimize the simultaneous extraction and purification of paeoniflorin and albiflorin from Radix Paeoniae Rubra. Methods: The optimal extraction conditions for achieving the highest total yield of paeoniflorin and albiflorin were determined using orthogonal array design. Further, the purification of paeoniflorin-albiflorin extract by microporous resin adsorption was explored. Results: The optimal extraction conditions were 70% ethanol as extraction solvent at a material-to-liquid ratio of 1:8 (g/mL) for reflux extraction repeated three times for 2 h each time. The optimal purification was achieved on AB-8 macroporous adsorption resin column with a diameter/height ratio of 1:8 under the following conditions: sample loading amount of 4.4 BV, washing with 3 BV water and eluting with 6 BV 40% ethanol at a flow rate of 1.5 BV/h. Conclusion: The optimized extraction process is simple and practical and can provide high purity of total paeony glycoside (TPG, mainly paeoniflorin and albiflorin).

Key words: Radix Paeoniae Rubra, extraction, purification, macroporous adsorption resin

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