食品科学 ›› 2011, Vol. 32 ›› Issue (12): 133-138.doi: 10.7506/spkx1002-6630-201112030

• 工艺技术 • 上一篇    下一篇

大孔吸附树脂分离纯化洋葱皮黄酮的研究

徐怀德,陈 佳,*,包 蓉,刘 坤,李 晋   

  1. 1. 西北农林科技大学食品科学与工程学院
    2.西北农林科技大学林学院
  • 出版日期:2011-06-25 发布日期:2011-06-10

Separation and Purification of Flavonoids from Onion Peel by Macroporous Resin Adsorption

XU Huai-de1,CHEN Jia1,*,BAO Rong2,LIU Kun1,LI Jin1   

  1. 1. College of Food Science and Engineering, Northwest A&F University, Yangling 712100, China; 2. College of Forestry, Northwest A&F University, Yangling 712100, China
  • Online:2011-06-25 Published:2011-06-10

摘要: 以黄酮含量为指标,比较6种大孔吸附树脂对洋葱皮黄酮的吸附和解吸效果。通过静态吸附与解吸实验,筛选出效果较好的X-5树脂进行动态实验研究。结果表明:X-5树脂纯化洋葱皮黄酮的工艺条件为吸附流速2mL/min、上样溶液pH5.0、上样液质量浓度0.5mg/mL、50mL 80%乙醇作为洗脱液,洗脱流速lmL/min。经X-5树脂纯化后,洋葱皮黄酮纯度从7.95%提高到80.78%。三次重结晶后其纯度可达94.5%。该方法简单可行,纯化效果好,适合于工业化生产。

关键词: 大孔吸附树脂, 分离, 纯化, 洋葱皮, 黄酮

Abstract: The characteristics of adsorption and desorption of onion peel flavonoids on six kinds of macroporous resins were compared. Static adsorption and desorption experiments indicated that X-5 resin was the best resin for the separation and purification of flavonoids from onion peel. The optimal process parameters for purifying onion peel flavonoids with X-5 resin were sample loading flow rate of 0.5 mg/mL, sample pH 5.0 and 50 mL of 80% ethanol of as eluent at a flow rate of 1.0 mL/min. After the purification, the purity of flavonoids from onion peel was increased from 7.95% to 80.78% and could reach up to 94.5% after further triple recrystallization. The developed method is simple and feasible for industrialization.

Key words: macroporous adsorption resin, separation, purification, onion peel, flavonoids

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