食品科学 ›› 2011, Vol. 32 ›› Issue (12): 209-212.doi: 10.7506/spkx1002-6630-201112045

• 分析检测 • 上一篇    下一篇

桑椹总RNA抽提方法的比较

周向红,易乐飞,王 萍*   

  1. 淮海工学院海洋学院
  • 出版日期:2011-06-25 发布日期:2011-06-10
  • 基金资助:
    教育部大豆重点实验室开放课题(SB08A03)

Comparison of Methods for Total RNA Extraction from Mulberry Fruit

ZHOU Xiang-hong,YI Le-fei,WANG Ping*   

  1. School of Marine Science and Technology, Huaihai Institute of Technology, Lianyungang 222005, China
  • Online:2011-06-25 Published:2011-06-10

摘要: 为对比CTAB改良法、E.Z.N.A.TM总RNA抽提试剂盒Ⅱ、EZ-10柱式总RNA抽提试剂盒和异硫氰酸胍法4种方法抽提桑椹总RNA的效果,对该4种方法抽提后的总RNA进行纯度、得率和完整性检测以及进一步的RT-PCR检测。结果显示:CTAB改良法和E.Z.N.A.TM总RNA抽提试剂盒Ⅱ均适用于桑椹总RNA抽提,获得的总RNA纯度高、得率高、完整性好,完全可用于后续的分子生物学实验;但两者相比,CTAB改良法成本更低,操作更简单。

关键词: 多糖, 桑椹, 总RNA, 抽提, 方法比较, 多酚

Abstract: Four methods, including modified CTAB, E.Z.N.A.TM Total RNA KitⅡ, EZ-10 Span Column Total RNA Isolation Kit and guanidine thiocyanate, were evaluated for their performance for isolating total RNA from mulberry fruit by comparing total RNA yield, purity and completeness as well as RT-PCR results. The results showed that modified CTAB method and E.Z.N.A.TM Total RNA KitⅡ were adequate for extracting total RNA from mulberry fruit. The acquired total RNA had a good quality and could be absolutely suitable for being used for further molecular biology experiments. Besides, the modified CTAB method was more economical and easier to operate than E.Z.N.A.TM Total RNA KitⅡ.

Key words: mulberry fruit, total RNA, extraction, comparison, polyphenol, polysaccharide

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