食品科学 ›› 2011, Vol. 32 ›› Issue (12): 213-215.doi: 10.7506/spkx1002-6630-201112046

• 分析检测 • 上一篇    下一篇

离子色谱法测定地沟油和食用油中氯离子含量

魏益华,张金艳,戴廷灿,涂田华,罗林广   

  1. 1.江西省农业科学院农产品质量安全与标准研究所 2.农业部肉及肉制品质量监督检验测试中心
  • 出版日期:2011-06-25 发布日期:2011-06-10
  • 基金资助:
    江西省农业科学院创新基金资助项目(2010CQN006)

Determination of Chlorine in Hogwash Oil and Edible Oil by Ion Chromatography

WEI Yi-hua1,2,ZHANG Jin-yan1,DAI Ting-can1,TU Tian-hua1,LUO Lin-guang1,2   

  1. 1. Institute for Quality & Safety and Standards of Agricultural Products, Jiangxi Academy of Agricultural Sciences, Nanchang 330200, China; 2. Supervision, Inspection and Testing Center for Quality of Meat-products, Ministry of Agriculture, Nanchang 330200, China
  • Online:2011-06-25 Published:2011-06-10

摘要: 样品采用氧化钙为溶剂,在恒温马弗炉中550℃加热4h,冷却后用水超声浸提,过On Guard NaⅡ SPE柱除去处理液中金属离子,用离子色谱法测定其氯离子含量。结果表明:地沟油中氯离子含量远高于食用油中氯离子含量。离子色谱条件:分析柱IonPac AS19(4mm×250mm),KOH淋洗液30.0mmol/L,流速1.0mL/min,进样体积500μL,外标峰面积定量。该方法对氯离子的检出限为0.004mg/L,在0.004~0.8mg/L范围内具有良好线性关系(r=0.9992),平均加标回收率为88%,相对标准偏差为(RSD)为3.1%。该法操作简便,快速灵敏、准确。

关键词: 离子色谱法, 地沟油, 食用油, 氯离子

Abstract: An ion chromatography method was presented to determine chlorine in hogwash oil and edible oil. Oil samples were ashed in muffle furnace at 550 ℃for 4 h with calcium oxide, cooled down, extracted with water in an ultrasonic field, and purified on an On Guard Na Ⅱ SPE column for eliminating metal ions before ion chromatographic analysis. The chromatographic separation was carried out on an IonPac AS19 (4 mm× 250 mm) column using 30.0 mmol/L KOH as mobile phase at a flow rate of 1.0 mL /min. The injection volume was 500μL. The external standard method was used to quantify chlorine. The detection limit of the method was 0.004 mg/L and a good linear relationship was observed over the range from 0.004 to 0.8 mg/L (r = 0.9992). The average recovery rate and RSD were 88% and 3.1%, respectively. This method proved easy to use, rapid, sensitive and accurate.

Key words: ion chromatography, hogwash oil, edible oil, chlorine

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