食品科学 ›› 2018, Vol. 39 ›› Issue (8): 97-102.doi: 10.7506/spkx1002-6630-201808016

• 成分分析 • 上一篇    下一篇

直接进样串联质谱法快速筛查水产副产物中ω-3脂肪酸链磷脂

崔益玮1,2,俞喜娜1,2,李诗言3,戴志远1,2,陈康1,2,沈清1,2,*   

  1. (1.浙江工商大学海洋食品研究院,浙江?杭州 310012;2.浙江省水产品加工技术研究联合重点实验室,浙江?杭州 310012;3.浙江省水产质量检测中心,浙江?杭州 310023)
  • 出版日期:2018-04-25 发布日期:2018-04-17
  • 基金资助:
    国家自然科学基金青年科学基金项目(31601542);浙江省基础公益研究项目(LGN18C200001); 浙江省重点研发计划项目 (2017C03041)

Direct Injection Tandem Mass Spectrometry for Fast Screening of Phospholipids with ω-3 Fatty Acyl Chains in Aquatic Byproducts

CUI Yiwei1,2, YU Xina1,2, LI Shiyan3, DAI Zhiyuan1,2, CHEN Kang1,2, SHEN Qing1,2,*   

  1. (1. Institute of Seafood, Zhejiang Gongshang University, Hangzhou 310012, China; 2. State Key Laboratory of Aquatic Products Processing of Zhejiang Province, Hangzhou 310012, China;3. Aquatic Products Quality Inspection Center of Zhejiang Province, Hangzhou 310023, China)
  • Online:2018-04-25 Published:2018-04-17

摘要: 建立自下而上快速筛选含二十碳五烯酸(eicosapentaenoic acid,EPA)、二十二碳五烯酸(docosapentenoic acid,DPA)和二十二碳六烯酸(docosahexaenoic acid,DHA)链磷脂的新方法。通过针泵注射进样,以m/z 301、329和327为特征碎片离子峰进行母离子扫描(precursor ion scan,PreIS),采用去簇电压-100 V和碰撞电压-40 V,质谱扫描范围设定为350~1?150?Da,得到含EPA、DPA和DHA链磷脂质谱全谱图。经结构鉴定和归一法含量计算,结果表明:水产品加工副产物中磷脂种类丰富,共鉴定50?种EPA、DPA及DHA磷脂分子,其sn-1位置的脂肪酸链主要以C16:0、C18:1和C18:0为主,部分磷脂脂肪酸链不饱和度极高,如磷脂酰丝氨酸20:5/22:6和磷脂酰肌醇22:6/22:6等,以水产品加工副产物为原料开发ω-3脂肪酸链磷脂保健功能产品潜力较大。方法经验证,特征磷脂分子相对标准偏差不高于7.3%,PreIS模式下质谱数据稳定性较好。本方法可一步快速筛查样品中含ω-3脂肪酸链磷脂,理论上同样适用于其他生物样品,可为探索与发现磷脂新资源提供一定理论支持。

关键词: 直接进样, 串联质谱, 磷脂, 副产物, 多不饱和脂肪酸链

Abstract: In this study, a bottom-up method was established for the fast screening of eicosapentaenoic acid (EPA), docosapentenoic acid (DPA), and docosahexaenoic acid (DHA) acyl chain containing phospholipids. The oil extract from aquatic byproducts was injected using a syringe pump, and monitored under precursor ion scan (PreIS) mode with ?100 V declustering potential (DP) and ?40 V collision energy (CE) in the range of 350–1 150 Da. The product ions of m/z 301, 329 and 327 were used as the representative ions for EPA, DPA and DHA chains, respectively. The peaks of EPA, DPA and DHA were identified and quantified. The results showed that the aquatic byproducts contained many different types of EPA, DPA and DHA containing phospholipids. A total of 50 phospholipids of interest were identified, whose sn-1 positions were C16:0, C18:1 or C18:0 chains, including phosphatidylserine 20:5/22:6 and phosphatidylinositol 22:6/22:6, which were extremely unsaturated. Aquatic byproducts rich in EPA, DPA and DHA containing phospholipids therefore show great potential in the development of functional foods. The method was validated and found to be stable and precise as indicated by the relative standard deviations (RSDs) lower than 7.3%. In conclusion, this method is advantageous in fast screening of ω-3 fatty acyl chain containing phospholipids. Theoretically, this method is applicable to other biological samples as well, and can provide strong support for searching for new resources of ω-3 fatty acyl chain containing phospholipids.

Key words: direct injection, tandem mass spectrometry, phospholipids, aquatic byproducts, polyunsaturated fatty acyl chains

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