食品科学 ›› 2018, Vol. 39 ›› Issue (8): 191-197.doi: 10.7506/spkx1002-6630-201808030

• 工艺技术 • 上一篇    下一篇

青稞麸皮阿拉伯木聚糖的提取工艺优化及结构分析

徐中香,胡浩,李季楠,吴艳*   

  1. (上海交通大学农业与生物学院,上海 200240)
  • 出版日期:2018-04-25 发布日期:2018-04-17
  • 基金资助:
    “十三五”国家重点研发计划重点专项(2017YFD0400205)

Optimization of Extraction Process and Structural Analysis of Arabinoxylans from Hull-Less Barley Bran

XU Zhongxiang, HU Hao, LI Jinan, WU Yan*   

  1. (School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, China)
  • Online:2018-04-25 Published:2018-04-17

摘要: 以青稞麸皮为原料,利用响应面法优化NaOH溶液提取阿拉伯木聚糖的最佳工艺条件。在单因素试验的基础上,以料液比、提取温度、提取时间、NaOH质量浓度为自变量,以青稞麸皮阿拉伯木聚糖得率为响应值,作四因素三水平的响应面回归分析。得到最佳提取工艺为料液比1∶25(g/mL)、提取温度55?℃、提取时间3?h、NaOH质量浓度15?g/L,在此条件下阿拉伯木聚糖得率为14.31%,与理论值14.27%无显著差异。因此利用响应面优化得到的青稞麸皮阿拉伯木聚糖提取工艺稳定可行。采用高效凝胶渗透色谱、紫外吸收光谱、红外吸收光谱、高效离子交换色谱对提取的多糖进行纯度鉴定及初步结构分析,结果表明青稞麸皮阿拉伯木聚糖均一性较好;多糖中含有微量蛋白质,不含核酸,并且含有α、β-糖苷键结构;其主要单糖组成为阿拉伯糖、半乳糖、葡萄糖和木糖,各单糖对应物质的量比为11.61∶1∶3.62∶18,阿拉伯糖与木糖物质的量比值为0.645。

关键词: 响应面分析, 青稞麸皮, 阿拉伯木聚糖, 结构

Abstract: One-factor-at-a-time method and response surface methodology (RSM) were used in combination to optimize the extraction of arabinoxylans from hull-less barley bran with aqueous sodium hydroxide solution. The independent variables investigated were solid to solvent ratio, temperature, extraction time and sodium hydroxide concentration, while the response variable was the yield of arabinoxylans. Each independent variable was tested at three levels. The optimum conditions obtained were as follows: solid to solvent ratio, 1:25 (g/mL); extraction temperature, 55 ℃; extraction time, 3 h; and sodium hydroxide concentration, 15 g/L. Under the optimized conditions, the yield of arabinoxylans was 14.31%, which agreed well with the predicted value of 14.27%. Thus, the optimized process was stable and feasible. Analysis of purity and structural properties by high-performance gel permeation chromatography (HPGPC), UV absorption spectroscopy, IR spectroscopy, high-performance anion-exchange chromatography (HPAEC) showed that the arabinoxylans were homogeneous and contained a small amount of protein but no nucleic acid and they were mainly composed of four monosaccharide units: arabinose, galactose, glucose and xylose with a molar ratio of 11.61:1:3.62:18 ( molar ratio of arabinose to xylose = 0.645), which were linked via α and β-glycosidic bonds.

Key words: response surface methodology, hull-less barley bran, arabinoxylan, structure

中图分类号: