食品科学 ›› 2018, Vol. 39 ›› Issue (8): 295-301.doi: 10.7506/spkx1002-6630-201808046

• 安全检测 • 上一篇    下一篇

分散固相萃取-超高效液相色谱-串联高分辨质谱法测定新疆苹果中7 种农药残留

李俊芳,景伟文,李德强,潘乐   

  1. (新疆农业大学化学工程学院,新疆?乌鲁木齐 830052)
  • 出版日期:2018-04-25 发布日期:2018-04-17
  • 基金资助:
    新疆维吾尔自治区高校科研计划项目(XJEDU2016S043)

Determination of 7 Pesticide Residues in Xinjiang Grown Apple by Dispersive Solid-Phase Extraction Combined with Ultra Performance Liquid Chromatography-High Resolution Tandem Mass Spectrometry

LI Junfang, JING Weiwen, LI Deqiang, PAN Le   

  1. (College of Chemical Engineering, Xinjiang Agricultural University, ürümqi 830052, China)
  • Online:2018-04-25 Published:2018-04-17

摘要: 为快速得到新疆苹果中痕量农药信息,考察新疆苹果种植过程中常用的7?种农药,并针对这7?种常见农药建立了分散固相萃取-超高效液相色谱-串联高分辨质谱的分析方法,旨在快速筛查出可能的农药并同时定量。结果表明:7?种农药线性关系良好,相关系数均大于0.99。方法检出限为0.003~0.1?mg/kg,定量限为0.02~0.5?mg/kg,平均回收率为95%~119%之间,相对标准偏差小于10%(n=3)。该方法能够排除假阳性结果,快速高效地完成出口前新疆苹果中多农药残留的分析。

关键词: 超高效液相色谱-串联高分辨质谱, 分散固相萃取, 苹果, 多农药残留

Abstract: To quickly obtain information about trace pesticides in Xinjiang grown apple, we investigated 7 commonly used pesticides during apple planting, and established a dispersive solid phase extraction combined with ultra performance liquid chromatography-high resolution tandem mass spectrometry (UPLC-HRMS/MS) method to quickly screen and quantitatively analyze the target pesticides. The results showed that good linearity was observed for all 7 analytes with correlation coefficients above 0.99. The limits of detection (LODs) were in the range of 0.003–0.1 mg/kg and the limits of quantification (LOQs) ranged between 0.02 and 0.5 mg/kg. Average recoveries were in the range of 95%–119% with relative standard deviation (RSD) less than 10% (n = 3). The method allowed for the rapid multi-residue analysis of pesticides in Xinjiang grown apples to be exported without any false-positive results.

Key words: ultra performance liquid chromatography-high resolution tandem mass spectrometry (UPLC-HRMS/MS), disperse solid phase extraction (d-SPE), apple, multi-residual pesticides

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