食品科学 ›› 2011, Vol. 32 ›› Issue (13): 40-44.doi: 10.7506/spkx1002-6630-201113010

• 基础研究 • 上一篇    下一篇

5种野生多孔菌提取物的抗氧化活性

杨建1,2,袁海生1,*,曹云1,2   

  1. 1.森林与土壤生态国家重点实验室,中国科学院沈阳应用生态研究所 2.中国科学院研究生院
  • 出版日期:2011-07-15 发布日期:2011-07-02
  • 基金资助:
    国家自然科学基金项目(30910103907;30700004)

Antioxidant Activities of Different Solvent Extracts of Phellinus Fruiting Bodies from Five Species

YANG Jian1,2,YUAN Hai-sheng1,*,CAO Yun1,2   

  1. 1. State Key Laboratory of Forest and Soil Ecology, Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang 110164, China;2. Graduate University of Chinese Academy of Sciences, Beijing 100049, China
  • Online:2011-07-15 Published:2011-07-02

摘要: 以BHT、TBHQ和Trolox 3种合成抗氧化剂为阳性对照,运用DPPH法和ABTS法对瓦宁木层孔菌(Phellinus vaninii)、喜马拉雅木层孔菌(Phellinus himalayensis)、尖小木层孔菌(Phellinidium aciforum)、乌苏里黑孔菌(Nigroporus ussuriensis)和桦褐孔菌(Inonotus obliquus)5种野生多孔菌子实体的乙酸乙酯提取物(EAE)和无水乙醇提取物(AEAE)的抗氧化活性进行测定和评价。结果显示:5种菌的EAE和AEAE清除自由基能力具有显著差异(P<0.05),并且每种菌AEAE清除自由基活性均高于其EAE;瓦宁木层孔菌AEAE清除DPPH自由基的活性最高,其IC50值为60.74mg/L,低于BHT(IC50值为90.51mg/L);清除ABTS+ ·能力最强的也是瓦宁木层孔菌AEAE,其IC50值为32.26mg/L。因此瓦宁木层孔菌(杨黄)具有很强的抗氧化能力。

关键词: 多孔菌, 乙酸乙酯提取物, 无水乙醇提取物, 抗氧化, 自由基, 瓦宁木层孔菌

Abstract: The antioxidant activities of ethyl acetate extracts (EAE) and absolute ethyl alcohol extracts (AEAE) from the fruiting bodies of Phellinus vaninii (PV), Phellinus himalayensis (PH), Phellinidium aciforum (PA), Nigroporus ussuriensis (NU) and Inonotus obliquus (IO) were evaluated by determining their scavenging rates against 1,1-diphenyl-2-picrylhydrazyl (DPPH) and 2,2-azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) free radicals. The antioxidants BHT, TBHQ and Trolox were used as positive controls. The results indicated that EAEs from the fruiting bodies of the five Phellinus species significantly differed from AEAEs in their free radical scavenging rate (P < 0.05). The free radical scavenging rate of AEAE from each Phellinus species was higher than that of EAE. The AEAE from PV exhibited the strongest free radical scavenging capacity with IC50 of 60.74 mg/L for DPPH free radicals and 32.26 mg/L for ABTS free radicals, respectively. However, the IC50 of BHT was 90.51 mg/L for DPPH free radicals. Therefore, the fruiting bodies of PV have stronger antioxidant capacity, and can be used in the food industry as a promising natural substitute for synthetic antioxidants.

Key words: Phellinu, ethyl acetate extract, ethyl alcohol extract, antioxidant, free radical, Phellinus vaninii

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