食品科学 ›› 2011, Vol. 32 ›› Issue (13): 45-48.doi: 10.7506/spkx1002-6630-201113011

• 基础研究 • 上一篇    下一篇

药桑椹花青素体外抗氧化作用

江岩,郑力,克热木江·吐尔逊江   

  1. 新疆大学生命科学与技术学院
  • 出版日期:2011-07-15 发布日期:2011-07-02
  • 基金资助:
    新疆维吾尔自治区自然科学基金项目(2011211B10);新疆大学博士科研启动基金项目(BS080121)

Antioxidant Activity of Anthocyanins from Morus nigra L. in vitro

JIANG Yan,ZHENG Li, Kerimjan·TURSUNJAN   

  1. College of Life Science and Technology, Xinjiang University, Urumqi 830046, China
  • Online:2011-07-15 Published:2011-07-02

摘要: 研究药桑椹花青素(anthocyanins from Morus nigra L.,AMNL)的体外抗氧化作用,并与白桑椹花青素(anthocyanins from Morus alba L.,AMAL)和黑桑椹花青素(anthocyanins from Morus alba Linn. var. tatarica,AMALV)的体外抗氧化作用进行比较。以芦丁和VC为对照,测定AMNL对二苯代苦味酰肼自由基(DPPH自由基)、超氧阴离子自由基(O2 ·)、羟自由基( ·OH)和烷基自由基的清除能力,同时测定其体外总抗氧化能力。结果表明,AMNL对DPPH自由基的清除能力(IC50=9.07mg/L) 显著高于AMAL与AMALV,弱于芦丁和VC。AMNL对O2 · (IC50=2.82mg/L)和 ·OH(IC50=7.77mg/L)的清除能力不仅明显高于AMAL与AMALV,而且清除效果也优于芦丁和VC。各桑椹花青素对烷基自由基清除率均低于50%。AMNL总抗氧化能力分别是AMAL、AMALV及芦丁的11.8倍、2.6倍及1.3倍,但低于VC。

关键词: 药桑椹, 花青素, 体外抗氧化, DPPH法

Abstract: The antioxidant activity of anthocyanins from Morus nigra L. (AMNL) in vitro was determined and compared with that of anthocyanins from Morus alba L. (AMAL) and Morus alba Linn. var. tatarica. (AMALV). The antioxidant evaluation was performed by 1,1-diphenyl-2-picryl-hydrazyl (DPPH), superoxide anion, hydroxyl and alkyl free radical scavenging assays and total antioxidant capacity (TOA) assay using rutin and VC as controls. The results showed that AMNL had stronger scavenging effect on DPPH free radicals with IC50 of 9.07 mg/L than AMAL and AMALV, but weaker scavenging effect on DPPH free radicals than rutin and vitamin C. Similarly, AMNL had more powerful scavenging effect on superoxide anion free radicals with IC50 of 2.82 mg/L and hydroxyl free radicals with IC50 of 7.77 mg/L than AMAL, AMALV, rutin and VC. However, anthocyanins from all the three cultivars of mulberry had a scavenging rate of less than 50% against alkyl free radicals. The total antioxidant capacity of AMNL revealed 11.8-, 2.6- and 1.3-fold enhancement when compared with AMAL, AMALV and rutin, but was lower than that of vitamin C.

Key words: Morus nigra L., anthocyanins, antioxidation in vitro, 1,1-diphenyl-2-picryl-hydrazyl (DPPH) free radical scavenging assay

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