食品科学 ›› 2011, Vol. 32 ›› Issue (13): 198-200.doi: 10.7506/spkx1002-6630-201113043

• 生物工程 • 上一篇    下一篇

南极磷虾自溶酶性质的初步研究

杭虞杰1,2,李学英2,杨宪时2,*,郭全友2   

  1. 1. 上海理工大学医疗器械与食品学院
    2.中国水产科学研究院东海水产研究所
  • 出版日期:2011-07-15 发布日期:2011-07-02
  • 基金资助:
    国家“863”计划项目(2010AA0922336001); 中央级公益性科研院所基本科研业务费专项资金(中国水产科学研究院东海水产研究所)资助项目(2009T05)

Properties of Autolysis Enzymes from Antarctic Krill

HANG Yu-jie1,2,LI Xue-ying2,YANG Xian-shi2,*,GUO Quan-you2   

  1. 1. School of Medical Instrument and Food Engineering, University of Shanghai for Science and Technology, Shanghai 200093, China;2. East China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Shanghai 200090, China
  • Online:2011-07-15 Published:2011-07-02

摘要: 探讨南极磷虾自溶酶的最适pH值、最适温度以及抑制剂和金属离子对其活性的影响。结果表明:南极磷虾自溶酶主要含有3种酶,最适pH值分别为3.0、6.0、8.0;各最适pH值条件下最适温度分别为40、50、50~60℃之间。苯甲基磺酰氟(PMSF)和大豆胰蛋白酶抑制剂(SBTI)对南极磷虾自溶酶活性有较强的抑制作用,抑制范围分别在pH3.0~9.0之间和pH6.0~10.5之间。Cu2+和Ba2+对自溶酶活性的抑制作用较强,Ca2+对自溶酶活性几乎没有抑制作用,而Mg2+对自溶酶活性有一定的激活作用。

关键词: 南极磷虾, 自溶酶, 性质研究

Abstract: The optimal pH and temperature of autolysis enzymes from Antarctic krill were investigated in this paper. Meanwhile, the effects of inhibitors and metal ions on the activities of autolysis enzymes from Antarctic krill were also explored. The results showed that autolysis enzymes from Antarctic krill were mainly composed of three enzymes with respective optimal reaction pH of 3.0, 6.0 and 8.0. In addition, the optimal reaction temperatures of the three enzymes were determined to be 40, 50 ℃ and 50-60 ℃, respectively. phenylmethyl sulfonyl fluoride (PMSF) and soybean trypsin inhibitor (SBTI) exhibited an obvious inhibition effect on autolysis enzymes from Antarctic krill at a pH ranging from 3.0 to 9.0 and from 6.0 to 10.5, respectively. Moreover, Cu2+ and Ba2+ could strongly inhibit the activities of the autolysis enzymes although Ca2+ did not reveal inhibition effect. In contrast, the autolysis enzymes were activated by Mg2+.

Key words: Antarctic krill, autolysis enzyme, characterization

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