食品科学 ›› 2011, Vol. 32 ›› Issue (13): 205-208.doi: 10.7506/spkx1002-6630-201113045

• 生物工程 • 上一篇    下一篇

产α-葡萄糖苷酶抑制剂桑树内生细菌的分离及鉴定

武婷婷,刘万振,生吉萍,王正荣,申琳*   

  1. 中国农业大学食品科学与营养工程学院
  • 出版日期:2011-07-15 发布日期:2011-07-02
  • 基金资助:
    国家公益性行业(农业)科研专项经费资助项目(200803033)

Isolation and Identification of a Strain ofα-Glucosidase Inhibitor-producing Endophytic Bacteria from Mulberry

WU Ting-ting,LIU Wan-zhen,SHENG Ji-ping,WANG Zheng-rong,SHEN Lin*   

  1. College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China
  • Online:2011-07-15 Published:2011-07-02

摘要: 从桑树内生细菌中筛选得到一株产α-葡萄糖苷酶抑制剂的内生细菌S-J-X-4,通过生理生化及分子生物学鉴定,初步鉴定其为萎缩芽孢杆菌(Bacillus atrophaeus)。对α-葡萄糖苷酶抑制剂的来源进行了初步研究,结果表明发酵液中的活性物质为萎缩芽孢杆菌的一种胞外分泌物。

关键词: 内生菌, 鉴定, α-葡萄糖苷酶抑制剂, 桑树, 内生细菌

Abstract: We isolated a strain of endophytic bacteria capable of producingα-glucosidase inhibitor from mulberry and named it S-J-X-4. On the basis of physiological and biochemical characterization and 16S rDNA gene sequence, the strain was preliminarily identified to as Bacillus atrophaeus. S-J-X-4 was cultured in LB medium andα-glucosidase inhibitor was found in the fermentation broth as an extracellular product.

Key words: α-glucosidase inhibitor, mulberry, endophytic bacteria, identification

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