食品科学 ›› 2011, Vol. 32 ›› Issue (14): 156-160.doi: 10.7506/spkx1002-6630-201114032

• 工艺技术 • 上一篇    下一篇

四角蛤蜊天然提取物制备工艺参数优化

舒留泉,易小兰,徐文渊,姚兴存   

  1. 淮海工学院 江苏省海洋生物技术重点实验室
  • 出版日期:2011-07-25 发布日期:2011-06-18

Process Parameter Optimization for Extraction of Natural Extract from Mactra veneriformis

SHU Liu-quan,YI Xiao-lan,XU Wen-yuan,YAO Xing-cun   

  1. (Jiangsu Key Laboratory of Marine Biotechnology, Huaihai Institute of Technology, Lianyungang 222005, China)
  • Online:2011-07-25 Published:2011-06-18

摘要: 对四角蛤蜊软体部一般成分和氨基酸组成进行分析,结果(以干基计)粗蛋白和粗脂肪质量分数分别为60.14%和6.08%,17种氨基酸总量为48.09%,其中必需氨基酸质量分数为18.94%,占氨基酸总量的 39.38%,呈味氨基酸质量分数为22.73%,占氨基酸总量的47.27%。在单因素试验的基础上,以温度、加盐量、pH值为影响因素,利用响应面方法对四角蛤蜊天然提取物制备工艺进行优化,得到的最佳工艺参数为温度82.07℃、pH7.87、加盐量1.75%,提取率为54.78%。

关键词: 四角蛤蜊, 一般成分, 天然提取物, 响应面法

Abstract: The proximate chemical composition and amino acid composition of the soft part from Mactra veneriformis were analyzed. Based on dry weight, the contents of protein and lipid were 60.14% and 6.08%, respectively, and the content of 17 identified amino acids was determined to be 48.09%, and the content of essential amino acids (EAA) was 18.94% and of delicious amino acids (DAA) 22.73%, making up 39.38% and 47.27% of total amino acids, respectively. Natural extract (protein) extraction from the soft part from Mactra veneriformis was performed by homogenization with water, pH adjustment and extraction with NaCl. Based on one-factor-at-a-time experiments, response surface methodology (RSM) was employed to optimize the preparation process. The optimal extraction conditions were extraction temperature of 82.07 ℃, pH 7.87 and NaCl concentration of 1.75%. Under these conditions, the protein recovery reached up to 54.78%.

Key words: Mactra veneriformis, composition, natural extract, response surface methodology (RSM)

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