食品科学 ›› 2011, Vol. 32 ›› Issue (17): 133-136.doi: 10.7506/spkx1002-6630-201117026

• 基础研究 • 上一篇    下一篇

杜仲翅果油体外抗氧化能力研究

向志钢1,2, 李先辉1,2, 张永康2   

  1. 1. 吉首大学医学院 2. 吉首大学 植物资源保护与利用湖南省高校重点实验室
  • 发布日期:2011-08-30
  • 基金资助:
    植物资源与利用湖南省高校重点实验室开放基金课题(JSK201114)

Antioxidant Activity in vitro of Seed Oil from Eucommia ulmoides Oliver Key Fruits

XIANG Zhi-gang1,2,LI Xian-hui1,2, ZHANG Yong-kang2   

  1. (1. College of Medicine, Jishou University, Jishou 416000, China;2. Key Laboratory of Plant Resources Conservation and Utilization, College of Hunan Province,Jishou University, Jishou 416000, China)
  • Published:2011-08-30

摘要: 目的:研究杜仲翅果油体外抗氧化作用。方法:采用三氯乙酸法测定杜仲翅果油的还原能力, Fenton反应体系测定杜仲翅果油的羟自由基( ·OH)清除率,NBT光化还原法测定杜仲翅果油的超氧阴离子自由基(O2- ·)清除率,红细胞氧化溶血法反映杜仲翅果油的细胞膜保护作用,硫代巴比妥酸法测定肝匀浆丙二醛(MDA)相对含量以反映杜仲翅果油对脂质过氧化的影响,并与VC进行比较。结果:杜仲翅果油表现为有一定的还原能力,具有显著的清除O2- ·和 ·OH能力;能抑制小鼠肝组织MDA的生成,减少红细胞氧化溶血和肝组织自发性脂质过氧化,且成量效关系,质量浓度为9mg/mL时作用最强。结论: 杜仲翅果油具有体外抗氧化能力。

关键词: 杜仲翅果油, 抗氧化性, 自由基

Abstract: Objective: To study the antioxidant activity of seed oil from Eucommia ulmoides Oliver key fruits in vitro. Methods: The reducing power and superoxide anion radical scavenging rate of the oil were measured by trichloroacetic acid method and NBT photoreduction method, respectively. The scavenging rate against hydroxyl radicals generated in Fenton system was determined by NBT photoreduction method. The cell membrane-protecting effect was determined by oxidative hemolysis of erythrocytes and the inhibitory effect against lipid peroxidation was evaluated by determining the relative content of MDA in mouse liver homogenate. Vitamin C was used as the control. Results: The oil had strong reducing power so that it could remarkably eliminate superoxide anion free radicals and hydroxyl free radicals. In addition, the oil could also inhibit the generation of MDA in mouse liver homogenate, reduce H2O2-mediated oxidation hemolysis of erythrocytes and spontaneous lipid peroxidation of mouse liver tissue in a dose-dependent manner. The oil at the concentration of 9 mg/mL revealed the strongest effect. Conclusion: Seed oil from Eucommia ulmoides Oliver key fruits has antioxidant capability in vitro.

Key words: seed oil from Eucommia ulmoides Oliver key fruits, antioxidant capability, free radicals

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