食品科学 ›› 2011, Vol. 32 ›› Issue (17): 225-229.doi: 10.7506/spkx1002-6630-201117046

• 生物工程 • 上一篇    下一篇

鸡蛋卵转铁蛋白过敏原B细胞线性表位的预测研究

佟 平1,2,高金燕3,陈红兵1,2,*,李 欣1,2,简 姗1,2,张 银1,2   

  1. 1. 南昌大学食品科学与技术国家重点实验室
    2.南昌大学中德联合研究院 3.南昌大学生命科学与食品工程学院食品系
  • 发布日期:2011-08-30
  • 基金资助:
    国家自然科学基金项目(31060215);教育部新世纪优秀人才支持计划项目(NCET-08-0704); 南昌大学食品科学与技术国家重点实验室目标导向项目(SKLF-MB- 201002)

Prediction of B-cell Linear Epitopes in Egg Allergen Ovotransferrin

TONG Ping1,2,GAO Jin-yan3,CHEN Hong-bing1,2,*,LI Xin1,2,JIAN Shan1,2,ZHANG Yin1,2   

  1. (1. State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China; 2. Sino-German Joint Research Institute, Nanchang University, Nanchang 330047, China; 3. Department of Food Science, School of Life Sciences and Food Engineering, Nanchang University, Nanchang 330047, China)
  • Published:2011-08-30

摘要: 为预测鸡蛋过敏原卵转铁蛋白的B细胞线性表位,以GenBank中提供的鸡蛋卵转铁蛋白氨基酸序列为基础,分别用Jameson-Wolf法、Kyte-Doolittle法、Emini法和Karplus-Schulz法对鸡蛋卵转铁蛋白的抗原指数、亲水性、蛋白表面可及性及柔韧性进行预测,并用Chou-Fasman法和Deleage-Roux法预测其二级结构,综合分析以上预测结果,得出鸡蛋卵转铁蛋白可能的B细胞线性表位区。结果表明鸡蛋卵转铁蛋白存在7个潜在表位区,包括蛋白第174~181、215~222、231~240、288~294、332~344、415~429、547~555位可能为B细胞线性表位优势区域。该结果将有助于鸡蛋卵转铁蛋白B细胞表位的进一步精确定位。

关键词: 卵转铁蛋白, B细胞线性表位, 鸡蛋过敏, 预测

Abstract: In order to predict B-cell linear epitopes of egg allergen ovotransferrin (OVT), the antigen index, hydrophilicity, and surface accessibility and flexibility of egg OVT were predicted using Jameson-Wolf, Kyte-Doolittle, Emini and Karplus-Schulz methods, respectively. The secondary structure of OVT was predicted by Chou-Fasman and Deleage-Roux methods based on the amino acid sequence of egg OVT derived from Genbank. Based on the above comprehensive analysis, 7 possible B-cell linear epitope candidates in egg OVT were predicted to locate in the regions of 174-181, 215-222, 231-240, 288-294, 332-344, 415-429 and 547-555. These results are useful for fine mapping of B-cell epitopes in egg OVT.

Key words: ovotransferrin, B-cell linear epitope, egg allergy, prediction

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