食品科学 ›› 2011, Vol. 32 ›› Issue (17): 243-245.doi: 10.7506/spkx1002-6630-201117050

• 生物工程 • 上一篇    下一篇

高粱黑粉菌的分离鉴定

路秀丽1,姚艳飞2,王 莘2,*   

  1. 1. 吉林农业大学生命科学学院
    2.吉林农业大学生命科学院
  • 发布日期:2011-08-30
  • 基金资助:
    长春市科技局科技支撑计划项目(2008214)

Isolation and Identification of Sporisorium reilianum

LU Xiu-li1,YAO Yan-fei2,WANG Xin2,*   

  1. (1. College of Food Science and Engineering, Jilin Agricultural University, Changchun 130118, China; 2. College of Life Sciences, Jilin Agricultural University, Changchun 130118, China)
  • Published:2011-08-30

摘要: 目的:以“吉粮105A”高粱乌米为材料,进行高粱黑粉菌的分离鉴定。方法:利用单胞分离的方法从样品中分离获得纯菌株,对分离菌株进行形态学观察及ITS(internal transcribed spacer,ITS)序列测定。结果:分离得到的菌株在天然固体培养基中形成的菌落表面呈革质,边缘呈锯齿状,棕黑色,ITS扩增片段大小为721bp;利用DNAStar软件对序列进行比较分析,鉴定出所分离菌株为丝孢堆菌。结论:成功从高粱乌米中分离出一株高粱丝孢堆黑粉菌。

关键词: 黑粉菌, ITS, 分离, 鉴定

Abstract: Objective: To isolate and identify Sporisorium reilianum from sorghum cultivar Jiliang 105A . Methods: Single spores were isolated from samples. The morphology of isolated spores was examined and the internal transcribed spacer (ITS) was sequenced. Results: The strain grown on the natural solid culture medium revealed leather face as well as jagged and brownish-black edge. The amplified fragment length of ITS was 721 bp. Based on sequence alignment carried out using DNAStar software, the isolated strain was identified as Sporisorium reilianum. Conclusion: Sporisorium reilianum is successfully isolated and identified from Jiliang 105A .

Key words: Sporisorium reilianum, internal transcribed spacer (ITS), isolation, identification

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