食品科学 ›› 2011, Vol. 32 ›› Issue (17): 321-325.doi: 10.7506/spkx1002-6630-201117067

• 营养卫生 • 上一篇    下一篇

可溶性膳食纤维对高脂膳食大鼠血清短链脂肪酸的影响

胡 娟1,吴洪斌2,郑 刚1,赵国华1,3,*   

  1. 1. 西南大学食品科学学院
    2. 新疆农垦科学院农产品加工研究所 3. 重庆市农产品加工技术重点实验室
  • 发布日期:2011-08-30
  • 基金资助:
    新疆生产建设兵团博士基金项目(2009JC12);国家“863”计划项目(2011AA100805)

Effects of Soluble Dietary Fibers on Serum Short-chain Fatty Acids of High-fat-diet Rats

HU Juan1,WU Hong-bin2,ZHENG Gang1,ZHAO Guo-hua1,3,*   

  1. (1. College of Food Science, Southwest University, Chongqing 400715, China ; 2. Institute of Agricultural Products Processing, Xinjiang Academy of Agricultural and Reclamation Science, Shi hezi 832000, China; 3. Chongqing Key Laboratory of Agricultural Products Processing, Chongqing 400715, China)
  • Published:2011-08-30

摘要: 以SD大鼠为实验动物,饲喂高脂饲料,同时灌胃可溶性的燕麦β-葡聚糖、番茄皮膳食纤维和葡萄皮膳食纤维,喂养50d后取血,以中空纤维支撑液膜萃取-气相色谱法测定血脂实验大鼠血清的短链脂肪酸(short-chain fatty acids,SCFA)含量,探讨3种可溶性膳食纤维对高脂膳食大鼠血清SCFA的影响。研究发现,与高脂膳食模型大鼠相比,燕麦β-葡聚糖对血清丙酸、丁酸含量有显著降低作用(P<0.01),分别下降了76.09%和55.79%;与高脂膳食模型大鼠相比,番茄皮膳食纤维组大鼠的血清乙酸含量有显著性提高(P<0.01),提高了81.56%,对血清戊酸含量有显著降低作用 (P<0.01),下降了62.63%;与高脂膳食模型大鼠相比,葡萄皮膳食纤维对血清乙酸含量有显著提高作用(P<0.01),提高了97.60%,但对血清异丁酸、异戊酸、戊酸有显著降低作用(P<0.01),其分别下降了66.59%、39.33%和63.82%。

关键词: 番茄, 葡萄, β-葡聚糖, 可溶性膳食纤维, 中空纤维支撑液膜, 血清, 短链脂肪酸

Abstract: In order to explore the effects of soluble dietary fibers on serum short-chain fatty acids (SCFA) of SD rats fed high fat diet, the rats were orally administered with soluble oat β-glucan, soluble tomato skin dietary fiber and soluble grape skin dietary fiber for 50 consecutive days. The serum SCFAs of rats were extracted by hollow fiber-supported liquid membrane extraction and quantitatively determined by gas chromatography. Compared with the model rats, soluble oat β-glucan could result in a significant decrease of propionate acid and n-butyric acid by 76.09% and 55.79%, respectively (P<0.01); soluble dietary fiber from tomato peel could result in an increase of acetic acid by 81.56% (P<0.01) and a significant reduction of n-valeric acid by 62.63% (P<0.01); soluble dietary fiber from grape peel could significantly increase acetic acid by 97.60% (P<0.01) and significantly reduce i-butyric acid, i-valeric acid and n-valeric acid by 66.59%, 39.33% and 63.82%, respectively (P<0.01).

Key words: tomato, grape, β-glucan, soluble dietary fiber, hollow fiber-supported liquid membrane extraction, serum, short-chain fatty acid

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