食品科学 ›› 2018, Vol. 39 ›› Issue (10): 78-83.doi: 10.7506/spkx1002-6630-201810013

• 食品化学 • 上一篇    下一篇

香榧不同部位提取物的抗氧化和酶抑制活性比较分析

张露1,刘鹏飞1,涂宗财1,2,*,谢星2,张恕雅1,沙小梅1,王辉2,戴花剑1   

  1. (1.江西师范大学生命科学学院,江西?南昌 330022)2.南昌大学 食品科学与技术国家重点实验室,江西?南昌 330047)
  • 出版日期:2018-05-25 发布日期:2018-05-15
  • 基金资助:
    江西省青年科学基金项目(20171BAB214037);江西省教育厅项目(GJJ150331)

Comparison of Antioxidant Activity and Enzyme Inhibitory Activity of Different Plant Parts of Torreya grandis cv. Merrillii

ZHANG Lu1, LIU Pengfei1, TU Zongcai1,2,*, XIE Xing2, ZHANG Shuya1, SHA Xiaomei1, WANG Hui2, DAI Huajian1   

  1. (1. College of Life Science, Jiangxi Normal University, Nanchang 330022, China; 2. State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China)
  • Online:2018-05-25 Published:2018-05-15

摘要: 通过分析香榧的叶、茎、假种皮、种壳和种仁提取液中总酚、总黄酮和缩合单宁的含量,及其自由基清除能力、α-葡萄糖苷酶和黄嘌呤氧化酶活性抑制能力,比较香榧不同部位活性成分含量和抗氧化能力的差异,间接证明其在降血糖和预防痛风中的应用潜力。结果表明:香榧不同部位提取液的主要活性成分、抗氧化和α-葡萄糖苷酶活性抑制能力存在显著差异(P<0.05)。香榧仁和香榧叶分别含有最高的总酚和缩合单宁含量,分别为25.28?mg?GAE/g和1.10?mg?CE/g,而香榧茎和香榧假种皮中的黄酮含量最高(P>0.05)。生物活性分析显示,香榧仁具有最高的DPPH自由基清除能力、ABTS+·清除能力、α-葡萄糖苷酶活性抑制能力和黄嘌呤氧化酶活性抑制能力,且其α-葡萄糖苷酶活性抑制能力(IC50=0.14?mg/mL)高于阳性对照品阿卡波糖(IC50=1.29?mg/mL)。因此,香榧仁是优于香榧叶、茎、假种皮和种壳的天然抗氧化剂、α-葡萄糖苷酶和黄嘌呤氧化酶抑制剂资源。

关键词: 香榧, 活性成分, 抗氧化, α-葡萄糖苷酶, 黄嘌呤氧化酶

Abstract: This study aimed to compare the phytochemical constituents, antioxidant activity, anti-diabetes activity and anti-gout potential of the leaf, stem, aril, seed shell and seed kernel of Torreya grandis cv. Merrillii by analyzing the contents of phenolics, flavonoids and condensed tannins, as well as radical scavenging ability and inhibitory activities against α-glucosidase and xanthine oxidase. The results indicated that the main bioactive constituents, antioxidant activity and α-glucosidase inhibitory activity of different plant parts varied significantly (P < 0.05). The kernel and leaf extracts had the highest total phenolic content (25.28 mg GAE/g) and condensed tannins content (1.10 mg CE/g), respectively. The highest content of flavonoids was found in the stem and aril extracts (P > 0.05). Bioactivity analysis revealed that the kernel extract showed the strongest 1,1-diphenyl-2-picrylhydrazyl (DPPH) scavenging ability, 2,2’-azinobis-(3-ethylbenzthiazoline-6-sulphonate) (ABTS+·) scavenging ability, inhibitory activities against α-glucosidase and xanthine oxidase, and its α-glucosidase inhibitory activity (IC50 = 0.14 mg/mL) was much higher than that of the positive control acarbose (IC50 = 1.29 mg/mL). Thus, it can be concluded that the seed kernel of Torreya grandis cv. Merrillii is a better resource of natural antioxidants and inhibitors of both α-glucosidase and xanthine oxidase than its leaf, stem, aril and seed shell.

Key words: Torreya grandis cv. Merrillii, bioactive constituents, antioxidant activity, α-glucosidase, xanthine oxidase

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