食品科学 ›› 2011, Vol. 32 ›› Issue (18): 91-94.doi: 10.7506/spkx1002-6630-201118020

• 工艺技术 • 上一篇    下一篇

复合酶法制备葛根多孔淀粉

江慧娟,黄赣辉*   

  1. 南昌大学 食品科学与技术国家重点实验室
  • 收稿日期:2018-06-20 修回日期:2018-06-20 出版日期:2011-09-25 发布日期:2011-09-24

Preparation of Porous Arrowroot Starch by Double Enzymatic Hydrolysis

JIANG Hui-juan,HUANG Gan-hui*   

  1. (State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China)
  • Received:2018-06-20 Revised:2018-06-20 Online:2011-09-25 Published:2011-09-24

摘要: 使用α-淀粉酶与糖化酶复合制备葛根多孔淀粉。通过单因素试验,对多孔淀粉吸油率进行考察,研究其品质特性随加酶量、酶配比、pH值、酶解时间和酶解温度等变化的规律。并由正交试验得出最佳工艺条件,当加酶量0.6%、酶解时间12h、pH5.0、酶解温度50℃、酶质量比(糖化酶:α-淀粉酶)3:1时吸油率最高(60%),且成孔效果良好。

关键词: 多孔淀粉, α-淀粉酶, 糖化酶, 酶解, 吸油率

Abstract: Porous arrowroot starch was prepared by simultaneous hydrolysis with α-amylase and glucoamylase. The effects of enzyme dosage, glucoamylase-to-α-amylase ratio, pH, hydrolysis time and hydrolysis temperature on the oil-absorbing capacity of porous arrowroot starch were investigated. The optimal hydrolysis conditions for preparing porous arrowroot starch were determined by orthogonal array design method to be 3:1 glucoamylase-to-α-amylase ratio, 0.6% total enzyme amount, pH 5.0, 12 h hydrolysis time and 50 ℃ hydrolysis temperature. Under these conditions, the maximum oil-absorbing rate of 3:1 and excellent pore formation were achieved.

Key words: porous starch, α-amylase, glucoamylase, hydrolysis, oil-absorbing capacity

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