食品科学 ›› 2011, Vol. 32 ›› Issue (18): 278-281.doi: 10.7506/spkx1002-6630-201118062

• 分析检测 • 上一篇    下一篇

微乳液直接进样-石墨炉原子吸收光谱法测定食用油中锰

陈尚龙,刘全德*,李 超,李向华   

  1. 徐州工程学院食品工程学院
  • 出版日期:2011-09-25 发布日期:2011-09-24
  • 基金资助:
    江苏省高校自然科学基金项目(10KJD550004)

Determination of Manganese in Edible Oil by GF-AAS with Direct Injection of Microemulsion

CHEN Shang-long,LIU Quan-de*,LI Chao,LI Xiang-hua   

  1. (College of Food Engineering, Xuzhou Institute of Technology, Xuzhou 221008, China)
  • Online:2011-09-25 Published:2011-09-24

摘要: 建立微乳液直接进样-石墨炉原子吸收光谱法测定食用油中锰的方法,研究微乳液形成的条件以及十二烷基硫酸钠的质量浓度、灰化温度、原子化温度对吸光度的影响。在单因素试验的基础上,通过正交试验确定了最佳测定条件为十二烷基硫酸钠的质量浓度4.0mg/mL、灰化温度900℃、原子化温度1900℃,在此条件下,测定食用油中锰的质量浓度为37.49ng/mL,精密度为1.86%,检出限为0.88ng/mL,加标平均回收率为101.8%,相对标准偏差为3.27%。微乳液直接进样-石墨炉原子吸收光谱法测定食用油中锰,不需要进行复杂的样品前处理,方法快速、准确、无污染,具有较高的实用价值。

关键词: 微乳液, 直接进样, 石墨炉原子吸收光谱法, 食用油,

Abstract: Graphite furnace atomic absorption spectrophotometry (GF-AAS) coupled with direct injection was used to establish a determination method for manganese in edible oil. The formation conditions of microemulsion and the effects of C12H25NaO4S concentration, ashing temperature and atomization temperature on absorbance were explored. Based on one-factor-at-a-time experiments, the optimal determination conditions were determined by orthogonal array design to be C12H25NaO4S concentration of 4.0 mg/mL, ashing temperature of 900 ℃ and atomization temperature of 1900 ℃. Under the optimal conditions, the content of manganese in edible oil was determined to be 37.49 ng/mL with a relative standard deviation of 1.86% and the detection limit of the method was 0.88 ng/mL. The average recovery rate for manganese in edible oil across three spike concentrations was 101.8% with a relative standard deviation of 3.27%. Therefore, this method is rapid, accurate and applicable without pollution and complex sample pretreatments.

Key words: microemulsion, direct injection, graphite furnace atomic absorption spectrophotometry, edible oil, manganese

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