食品科学 ›› 2011, Vol. 32 ›› Issue (20): 104-107.doi: 10.7506/spkx1002-6630-201120022

• 工艺技术 • 上一篇    下一篇

聚丙烯微孔膜固定化转谷氨酰胺酶在大豆乳清废水处理中的应用

马永强1,韩春然1,张 娜1,佟晓芳2,张毅方3,刘 颖1   

  1. 1.哈尔滨商业大学 黑龙江省食品科学与工程重点实验室 2.黑龙江省食品质量监督检验一站 3.哈高科大豆食品有限责任公司
  • 出版日期:2011-10-25 发布日期:2011-10-12
  • 基金资助:
    黑龙江省高校科技创新团队建设计划项目(2010td04);国家“863”计划项目(2008AA10Z303)

Application of Polypropylene Microporous Membrane Carrying Transglutaminase to Treat Soybean Whey Waste

MA Yong-qiang1,HAN Chun-ran1,ZHANG Na1,TONG Xiao-fang2,ZHANG Yi-fang3,LIU Ying1   

  1. (1. Key Laboratory of Food Science and Engineering of Heilongjiang Province, Harbin University of Commerce, Harbin 150076, China;2. Administration of Quality Supervision, Inspection of Heilongjiang, Harbin 150001, China; 3. Harbin Hi-tech Soybean Food Company, Harbin 150078, China)
  • Online:2011-10-25 Published:2011-10-12

摘要: 将固定化转谷氨酰胺酶酶膜应用到酶膜反应器中,对大豆乳清废水进行催化使其发生聚合并被截留,从而减轻大豆乳清废水对环境的污染,并确定其最佳影响条件,得出在最佳条件下进行处理的蛋白截留率为78.4%。对处理前后大豆乳清废水进行分析,其主要成分指标发生了很大变化,如蛋白质含量、生化需氧量(biochemical oxygen demand,BOD)值、化学需氧量(chemical oxygen demand,COD)值、灰分含量等指标较处理前发生显著下降。

关键词: 转谷氨酰胺酶, 甲基丙烯酸甲酯膜, 大豆乳清废水, 固定化酶, 催化

Abstract: In this study, we immobilized microbial transglutaminase (MTG) onto polypropylene microporous membrane by ozone activation and then evaluated its application to treat soybean whey waste with the purpose to recover proteins and decrease pollution. The results showed that under optimal technological conditions, MTG-conjugated polypropylene microporous membrane recovered 78.4% of proteins from soybean whey waste, and significantly decreased protein and ash contents, COD value and BOD value of soybean whey waste.

Key words: microbial transglutaminase, polypropylene microporous membrane, soybean whey wastewater, enzyme immobilization, atalysis

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