食品科学 ›› 2011, Vol. 32 ›› Issue (20): 146-150.doi: 10.7506/spkx1002-6630-201120030

• 分析检测 • 上一篇    下一篇

黄山贡菊高效液相色谱指纹图谱的建立

査芳芳1,高学玲1,*,邹敏亮2,何常明2,贺曼曼1   

  1. 1.安徽农业大学茶与食品科技学院 2.健士星生物技术研发(上海)有限公司
  • 出版日期:2011-10-25 发布日期:2011-10-12
  • 基金资助:
    国家星火计划项目(2010GA710027)

HPLC Fingerprinting of Huangshan Gongju Chrysanthemum (Chrysanthemum morifolium Ramat.)

ZHA Fang-fang1,GAO Xue-ling1,*,ZOU Min-liang2,HE Chang-ming2,HE Man-man1   

  1. (1. College of Tea & Food Science and Technology, Anhui Agricultural University, Hefei 230036, China; 2. Healthy-Star Biotech R&D Co. Ltd., Shanghai 200335, China)
  • Online:2011-10-25 Published:2011-10-12

摘要: 目的:研究黄山贡菊高效液相色谱指纹图谱的建立方法,为其质量控制和科学评价提供依据。方法:采用高效液相色谱法测定28种不同来源的菊花样品,用药典委员会颁布的中药色谱指纹图谱相似度评价系统2004A进行评价,建立共有模式。结果:建立了黄山贡菊的指纹图谱,确定了12个共有峰,各贡菊样品指纹图谱与对照指纹图谱相似度均在0.96以上,与其他菊花品种的相似度均小于0.86。结论:此方法简便、准确、重复性好,可用于黄山贡菊药材质量控制与评价。

关键词: 黄山贡菊, HPLC, 指纹图谱, 相似度

Abstract: Objective: To establish the HPLC fingerprint of Huangshan Gongju chrysanthemum (Chrysanthemum morifolium Ramat.) for its quality control and evaluation. Method: A total of 28 chrysanthemum samples of different geographical origins were determined by HPLC and evaluated using the Similarity Evaluation System for Chromatographic Fingerprints of Traditional Chinese Medicine to construct a common pattern. Results: Twelve common peaks were identified in the established fingerprints, each of which shared a similarity exceeding 0.96 with the control fingerprint and a similarity below 0.86 with other chrysanthemum varieties. Conclusion: The proposed method is simple, accurate, repeatable and suitable for quality control and evaluation of Huangshan Gongju chrysanthemum.

Key words: Huangshan Gongju chrysanthemum, high performance liquid chromatography (HPLC), chromatographic fingerprint, similarity

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