食品科学 ›› 2011, Vol. 32 ›› Issue (20): 160-163.doi: 10.7506/spkx1002-6630-201120033

• 分析检测 • 上一篇    下一篇

AccQ.Tag柱前衍生RP-HPLC法测定齿果酸模中的氨基酸含量

高向阳,刘 娜   

  1. 河南农业大学食品科学技术学院
  • 出版日期:2011-10-25 发布日期:2011-10-12
  • 基金资助:
    河南省重点学科建设项目(10466-X-082301)

Determination of Amino Acid Contents in the Whole Plant of Rumex dentatus by Reversed-Phase High-Performance Liquid Chromatography with AccQ ·Tag Pre-column Derivatization Method

GAO Xiang-yang,LIU Na   

  1. (College of Food Science and Technology, Henan Agricultural University, Zhengzhou 450002, China)
  • Online:2011-10-25 Published:2011-10-12

摘要: 为研究齿果酸模中氨基酸的种类和含量,探讨其营养特性和食用开发价值。以6-氨基喹啉-N-羟基琥珀酰亚胺基甲酸酯(AQC)为衍生试剂,以磷酸盐缓冲液为流动相A,乙腈溶液为流动相B进行梯度洗脱;在柱温37℃条件下用AccQ ·Tag进行柱前衍生,反相-高效液相色谱法用紫外检测器在波长248nm进行测定。结果表明:齿果酸模中含有17种氨基酸,干基总含量为252.35mg/g,有很好的食用开发前景。17种氨基酸线性回归方程的相关系数r在0.9995以上(n=5),测定的平均回收率为97.1%~102.6%,相对标准偏差RSD为0.3%~3.2%(n=3),测定结果令人满意。

关键词: 齿果酸模, AccQ.Tag柱前衍生, 反相-高效液相色谱法(RP-HPLC), 氨基酸

Abstract: Objective: To determine the composition and contents of amino acids in the whole plant of Rumex dentatus for further study of its nutritional characteristics and food value. Methods: Amino acids were derivatized by 6-aminoquinolyl-N-hydroxysuccinimidyl-carbamate (AQC), and then their derivatives were separated on a Waters 515 HPLC system with a column temperature of 37 ℃ using phosphate buffer as mobile phase A and aqueous acetonitrile as mobile phase B in the gradient elution mode and detected at 248 nm using a UV detector. Results: Totally 17 amino acids were found, and the total content of amino acids in samples was 252.35 mg/g. The correlation coefficients of the calibration curves for 17 amino acids were all above 0.9995 (n = 5). The average spike recoveries of 17 amino acids were between 97.1% and 102.6% with a relative standard deviation (RSD) between 0.3% and 3.26% (n = 3).

Key words: Rumex dentatus, AccQ ·Tag pre-column derivatization, reversed-phase high-performance liquid chromatography (RP-HPLC), amino acids

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