食品科学 ›› 2011, Vol. 32 ›› Issue (19): 12-15.doi: 10.7506/spkx1002-6630-201119003

• 基础研究 • 上一篇    下一篇

Cu2+、Co2+和Mg2+对杜鹃素与DNA相互作用的影响

付 鹏,张国文*,王 琳,胡明明   

  1. 南昌大学食品科学与技术国家重点实验室
  • 发布日期:2011-10-12
  • 基金资助:
    国家自然科学基金项目(21167013;31060210);江西省教育厅科技计划项目(GJJ11287); 江西省科技支撑计划项目(2010BSA17400)

Effects of Cu2+, Co2+ and Mg2+ on Interaction between Farrerol and DNA

FU Peng,ZHANG Guo-wen*,WANG Lin,HU Ming-ming   

  1. (State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China)
  • Published:2011-10-12

摘要: 在pH7.4的缓冲溶液中,采用紫外-可见、荧光光谱法和DNA熔点实验并结合荧光探针亚甲基蓝(MB),研究杜鹃素与小牛胸腺DNA的结合模式以及Cu2+、Co2+和Mg2+分别对结合模式的影响。结果表明:杜鹃素主要以嵌插模式与DNA结合。3种金属离子均能不同程度影响杜鹃素的吸收光谱和增强DNA-MB复合物的荧光强度。随着体系中Cu2+浓度的增加,DNA-杜鹃素复合物的结合常数呈现出先减小后增加的趋势,增加Mg2+的用量则使复合物的结合常数影响先增强然后缓慢减弱,而Co2+浓度的增大对复合物的结合常数影响较小。由此得出,Cu2+、Co2+和Mg2+对该DNA-杜鹃素结合的影响分别取决于金属离子与杜鹃素的结合能力以及与DNA的碱基和磷酸基团间的结合程度。

关键词: 杜鹃素, 小牛胸腺DNA, 金属离子, 荧光光谱, 嵌插作用

Abstract: The interaction between farrerol and calf thymus DNA (ctDNA) was explored by UV-visible spectrophotometry, fluorescence spectroscopy, and melting-point technique in combination with methylene blue (MB) competition in the presence of Cu2+, Co2+ and Mg2+ in physiological buffer (pH 7.4). The results revealed that farrerol could be intercalatively bound to DNA. Cu2+, Co2+ and Mg2+ had different effects on the absorption spectrum of farrerol and could increase the fluorescence intensity of DNA-MB complex. A gradual increase in Cu2+ concentration could result in an initial decrease followed by an increase in the binding constant of DNA-farrerol complex. In the presence of higher Mg2+ concentration, the binding constant of DNA-farrerol complex tended to initially increase and then decrease. However, Increasing Co2+ concentration revealed little effect on the binding constant of DNA-farrerol complex. Therefore, the effect of metal ions on the binding between farrerol and DNA mainly depends on the relative affinity between metal ions and farrerol, DNA basic groups or phosphate groups.

Key words: farrerol, calf thymus DNA, metal ions, fluorescence spectroscopy, binding mode

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