黄瓜多糖的体外抗氧化活性

doi:10.7506/spkx1002-6630-201119015

食品科学 ›› 2011, Vol. 32 ›› Issue (19): 70-74.doi: 10.7506/spkx1002-6630-201119015

黄瓜多糖的体外抗氧化活性

何念武,杨兴斌,田灵敏,赵燕

1.教育部药用资源与天然药物化学重点实验室，陕西师范大学食品工程与营养科学学院 2.第四军医大学药学院化学教研室

发布日期:2011-10-12
基金资助:
国家自然科学基金项目(30972054；20802091；31171678)

in vitro Antioxidant Activity of Cucumber Polysaccharides

HE Nian-wu1，YANG Xing-bin1，TIAN Ling-min1，ZHAO Yan2,*

(1. Key Laboratory for Medicinal Resource and Natural Pharmaceutical Chemistry, Ministry of Education, College of Food Engineering and Nutritional Science, Shaanxi Normal University, Xi’an 710062, China；2. Department of Chemistry, School of Pharmacy, Fourth Military Medical University, Xi’an 710032, China)

Published:2011-10-12

摘要/Abstract

摘要： 目的：测定黄瓜中总糖含量，分离制备黄瓜多糖并测定其糖醛酸含量、单糖组成以及评价其体外抗氧化活性。方法：采用苯酚-硫酸法测定黄瓜提取物中的总糖含量；用硫酸-咔唑法测定黄瓜多糖中的糖醛酸含量；采用1-苯基-3-甲基-5-吡唑啉酮(PMP)柱前衍生化高效液相色谱(HPLC)分析单糖组成；并在体外抗氧化评价体系研究黄瓜多糖对DPPH自由基、超氧阴离子自由基(O2－ ·)和羟自由基( ·OH)的清除活性以及总还原力(TRP)。结果表明：黄瓜多糖的总糖含量为63.5%，糖醛酸含量为10.6%。HPLC分析表明：黄瓜多糖由D-甘露糖、L-鼠李糖、D-葡萄糖醛酸、D-半乳糖醛酸、D-葡萄糖、D-木糖、D-半乳糖、L-阿拉伯糖等8种单糖组成，物质的量比为4.08:2.78:1.00:5.82:6.07:2.78:8.48:6.58。黄瓜多糖有明显的抗氧化活性，在质量浓度为20mg/mL时，对DPPH自由基、O2－ ·、 ·OH的清除率分别为92.31%、83.57% 和77.59%，并发现其有明显的还原能力。结论：黄瓜多糖是一种典型的杂多糖，具有较强的抗氧化活性。

关键词: 黄瓜, 多糖, 单糖组成, 自由基, 体外抗氧化活性

Abstract: Objective: To determine total carbohydrate content in cucumber, isolate polysaccharides from it and analyze uronic acid content, monosaccharide composition and in vitro antioxidant activity of extracts obtained. Methods: Phenol-sulfuric acid and sulfuric acid-carbazole methods were employed to evaluate total carbohydrate content and uronic acid content, respectively. In addition, high performance liquid chromatography (HPLC) based on pre-column derivatization with 1-phenyl-3-methyl-5-pyrazolone (PMP) was employed to analyze the monosaccharide composition of cucumber polysaccharides. Moreover, the scavenging activity of cucumber polysaccharides against 1,1-diphenyl-2-picrylhydrazyl (DPPH), superoxide anion (O2－ ·) and hydroxyl ( ·OH) free radicals and total reducing power (TRP) of cucumber polysaccharides were evaluated in vitro. Results: The total carbohydrate and uronic acid contents in cucumber polysaccharides were 63.5% and 10.6%, respectively. The monosaccharide composition of cucumber polysaccharides was made up of D-mannose (Man), L-rhamnose (Rha), D-glucuronic acid (GlcUA), D-galacturonic acid (GalUA), D-glucose (Glc), D-xylose (Xyl), D-galactose (Gal), L-arabinose (Ara) with a molar ratio of 4.08:2.78:1.00:5.82:6.07:2.78:8.48:6.58. Cucumber polysaccharides revealed high antioxidant activity, and the scavenging rates at the concentration of 20 mg/mL against DPPH, superoxide anion and hydroxyl free radicals were 92.31%, 83.57% and 77.59%, respectively. Moreover, cucumber polysaccharides also exhibited strong reducing power. Conclusion: Cucumber polysaccharides are typically heteropolysaccharides with stronger antioxidant activity.

Key words: cucumber, polysaccharide, monosaccharide composition, free radical, antioxidant activity in vitro

中图分类号:

O636.1

何念武，杨兴斌，田灵敏，赵燕. 黄瓜多糖的体外抗氧化活性[J]. 食品科学, 2011, 32(19): 70-74.

HE Nian-wu，YANG Xing-bin，TIAN Ling-min，ZHAO Yan. in vitro Antioxidant Activity of Cucumber Polysaccharides[J]. FOOD SCIENCE, 2011, 32(19): 70-74.

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黄瓜多糖的体外抗氧化活性

in vitro Antioxidant Activity of Cucumber Polysaccharides

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