食品科学 ›› 2011, Vol. 32 ›› Issue (19): 92-95.doi: 10.7506/spkx1002-6630-201119020

• 基础研究 • 上一篇    下一篇

桑黄菌多糖体外抗氧化作用

祝子坪,李 娜   

  1. 台州学院生命科学学院
  • 发布日期:2011-10-12
  • 基金资助:
    浙江教育厅计划项目(Y200906797)

Antioxidant Properties of Polysaccharides from Phellinus igniarius in vitro

ZHU Zi-ping, LI Na   

  1. (School of Life Science, Taizhou University, Taizhou 317000, China)
  • Published:2011-10-12

摘要: 采用化学模拟体系测定桑黄菌胞内多糖与胞外多糖体外抗氧化能力。结果表明:清除DPPH自由基时,胞内多糖的EC50值为1.09mg/mL,胞外多糖的EC50值为1.52mg/mL;清除 ·OH时,胞外多糖的EC50值为0.23mg/mL,胞内多糖的EC50值为0.78mg/mL;清除O2- ·时,胞外多糖的EC50值为20.31μg/mL,胞内多糖的EC50值为29.97μg/mL;螯合Fe2+时,胞内多糖的EC50值为1.36mg/mL,胞外多糖的EC50值为1.66mg/mL;实验范围内胞内多糖、胞外多糖还原能力与质量浓度呈很好的线性关系。可见桑黄菌多糖抗氧化能力有明显的量效关系,且胞外多糖与胞内多糖的抗氧化能力不同。

关键词: 桑黄菌, 多糖, 抗氧化

Abstract: The antioxidant properties of intracellular polysaccharides (IPS) and exopolysaccharides (EPS) from Phellinus igniarius were evaluated in vitro by chemical simulation. The results indicated that the EC50 of Phellinus igniarius IPS and EPS were 1.09 mg/mL and 1.52 mg/mL for scavenging DPPH radicals, 0.78 mg/mL and 0.23 mg/mL for scavenging hydroxyl radicals, 29.97μg/mL and 20.31μg/mL for scavenging superoxide anion radicals and 1.36 mg/mL and 1.66 mg/mL for chelating Fe2+, respectively. An excellent linear relationship between the reducing power and concentrations of both polysaccharides was observed in the tested concentration ranges. The antioxidant capacity of both polysaccharides was in a dose-dependent manner but showed an obvious difference.

Key words: Phellinus igniarius, polysaccharide, antioxidation

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