2007-2008西安地区鸡肉源沙门氏菌相关特性分析

doi:10.7506/spkx1002-6630-201119029

食品科学 ›› 2011, Vol. 32 ›› Issue (19): 130-136.doi: 10.7506/spkx1002-6630-201119029

2007-2008西安地区鸡肉源沙门氏菌相关特性分析

杨保伟,申进玲,席美丽,张秀丽崔生辉,王新,孟江洪

1. 西北农林科技大学食品科学与工程学院
2.河南省疾病预防控制中心 3.中国药品生物制品检定所

发布日期:2011-10-12
基金资助:
教育部长江学者讲座教授奖励计划项目(Z111020001)；西北农林科技大学留学回国人员基金项目(Z111020525)

Antibacterial Susceptibility and Subtypes of Salmonella Isolates from Retail Chicken in Xi，an in 2007－2008

YANG Bao-wei1，SHEN Jin-ling1，XI Mei-li1，ZHANG Xiu-li2，CUI Sheng-hui3，WANG Xin1，MENG Jiang-hong1

(1. College of Food Science and Engineering, Northwest A&F University, Yangling 712100, China； 2. Henan Center for Disease Control and Prevention, Zhengzhou 450016, China； 3. National Institute for the Control of Pharmaceutical and Biological Products, Beijing 100050, China)

Published:2011-10-12

摘要/Abstract

摘要： 目的：研究260株2007-2008年分离于西安市零售鸡肉中沙门氏菌的药敏性、血清型和基因型，以确保食品安全。方法：使用临床实验室标准化委员会(the Clinical and Laboratory Standards Institute，CLSI)推荐的琼脂稀释法测定沙门氏菌的药敏性；世界卫生组织(WHO)推荐的玻片凝集法测定沙门氏菌的血清型；使用美国疾病预防控制中心推荐的脉冲场凝胶电泳法确定沙门氏菌的DNA酶切图谱，BioNumerics软件分析电泳结果，确定沙门氏菌的基因型。结果：沙门氏菌对萘啶酮酸、环丙沙星、左氧氟沙星、加替沙星、氨苄西林、阿莫西林-克拉维酸、头孢哌酮、头孢曲松和头孢西丁的耐药率分别为81.4%、29.2%、22.4%、21.8%、34.6%、37.7%、32.6%、23.1%和1.9%。260株沙门氏菌共涵盖21个血清型，以肠炎沙门氏菌(Salmonella Enteritidis)、舒卜拉沙门氏菌(S. Shubra)、印第安纳沙门氏菌(S. Indiana)、鼠伤寒沙门氏菌(S. Typhimurium)、丘古沙门氏菌(S. Djugu)、德尔卑沙门氏菌(S. Derby)、维尔肖沙门氏菌(S. Virchow)、奥兹马森沙门氏菌(S. Othmarschen)和沙门氏菌Ⅱ(Salmonella Ⅱ)等比较常见。6.1%的舒卜拉沙门氏菌和22.6%的印第安纳沙门氏菌至少可耐15种抗生素，耐药性最强。4种最为常见的肠炎沙门氏菌、舒卜拉沙门氏菌、印第安纳沙门氏菌和鼠伤寒沙门氏菌均表现为基因型多样性，不同血清型沙门氏菌的流行状况随时间和地点的改变呈现出不同规律性。结论：西安市鸡肉源沙门氏菌的药敏性、血清型和基因型具有多样性特征，存在耐药现象。

关键词: 鸡肉, 沙门氏菌, 药敏性, 血清型, 基因型

Abstract: Objective: The antibacterial susceptibility, and serotype and genotype of 260 Salmonella isolates from retail chicken in Xi，an district of Shaanxi province were determined to explore the microbial safety of retail chicken. Methods: The antibacterial susceptibility was tested using agar dilution method recommended by the Clinical and Laboratory Standards Institute. Serotyping was performed using slide agglutination method according to WHO guidelines. DNA fingerprinting profiles of Salmonella were determined using pulse field gel electrophoresis (PFGE) developed by the US Centers for Disease Control and Prevention, and analyzed using the BioNumerics Software. Results: The drug resistance rates of 260 Salmonella isolates to nalidixic acid, ciprofloxacin, levofloxacin, gatifloxacin, ampicillin, amoxicillin-clavulanic acid, cefoperazone, ceftriaxone and cefoxitin were 81.4%, 29.2%, 22.4%, 21.8%, 34.6%, 37.7%, 32.6%, 23.1% and 1.9%, respectively. Twenty-one serotypes were identified in the Salmonella isolates, and the common serotypes were Salmonella Enteritidis, Salmonella Shubra, Salmonella Indiana, Salmonella Typhimurium, Salmonella Djugu, Salmonella Derby, Salmonella Virchow, Salmonella Othmarschen and Salmonella II, respectively. The isolates of serotypes Salmonella Indiana (22.6%) and Salmonella Shubra (6.1%) were resistant to at least 15 antibiotics, which were the most resistant ones. PFGE profiles of Salmonella Enteritidis, Salmonella Shubra, Salmonella Indiana and Salmonella Typhimurium isolates were genetically diverse. The epidemiological regularity of the different serotypes of Salmonella Exhibited differences along with sampling time and places. Conclusion: Salmonella isolates from retail chicken in Xi，an are diverse in serotypic and genotypic characteristics and many of them are resistant to multiple antibiotics commonly used in the treatment of humans and animals.

Key words: chicken, Salmonella, antibacterial susceptibility, serotype, genotype

中图分类号:

X172

杨保伟，申进玲，席美丽，张秀丽，崔生辉，王新，孟江洪. 2007-2008西安地区鸡肉源沙门氏菌相关特性分析[J]. 食品科学, 2011, 32(19): 130-136.

YANG Bao-wei，SHEN Jin-ling，XI Mei-li，ZHANG Xiu-li，CUI Sheng-hui，WANG Xin，MENG Jiang-hong. Antibacterial Susceptibility and Subtypes of Salmonella Isolates from Retail Chicken in Xi，an in 2007－2008[J]. FOOD SCIENCE, 2011, 32(19): 130-136.

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2007-2008西安地区鸡肉源沙门氏菌相关特性分析

Antibacterial Susceptibility and Subtypes of Salmonella Isolates from Retail Chicken in Xi，an in 2007－2008

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