黄孢原毛平革菌对大豆秸秆木质素降解的FTIR表征

doi:10.7506/spkx1002-6630-201119030

食品科学 ›› 2011, Vol. 32 ›› Issue (19): 137-140.doi: 10.7506/spkx1002-6630-201119030

黄孢原毛平革菌对大豆秸秆木质素降解的FTIR表征

王丽婷，徐伟*，张玉强

哈尔滨商业大学食品工程学院

发布日期:2011-10-12
基金资助:
黑龙江教育厅科技面上项目(12511130)

FTIR Characterization of Lignin Degradation Products from Soybean Straw Fermented by Phanerochaete chrysosporium Burdsall

WANG Li-ting，XU Wei*，ZHANG Yu-qiang

(College of Food Science and Engineering, Harbin University of Commerce, Harbin 150076, China)

Published:2011-10-12

摘要/Abstract

摘要： 研究黄孢原毛平革菌(Phanerochaete chrysosporium Burdsall)对大豆秸秆中木质纤维素的降解条件。结果显示：5g秸秆粉中添加合成培养液12mL、接种量为0.8mL(孢子悬液浓度8.6×106CFU/mL)、培养基初始pH4.5，发酵10d后，木质素降解率为45.96%。并采用傅里叶红外光谱分析(Fourier transform infrared spectroscopy，FTIR) 检测降解后秸秆中官能团的变化，发现与木质素相关的谱峰(1099cm-1、1057～1038cm-1)相对强度减小，与苯环相关的谱峰(1643～1608cm-1、1510～1508cm-1)相对强度增加，表明部分大分子木质素裂解成小分子木质素或木质素单体，对比其他谱峰相对强度的变化，发现木质素中苯环等环状化合物含量减少。

关键词: 大豆秸秆, 黄孢原毛平革菌, 木质素, FTIR

Abstract: The degradation conditions of soybean straw lignin by Phanerochaete chrysosporium fermentation were studied in this paper. The results showed that addition of 5 g of soybean straw power to 12 mL of synthetic medium for fermentation at an initial pH of 4.5 and an inoculum size of 0.8 mL (8.6 × 106 CFU/mL spore suspension concentration) for 10 d proved optimal and the resultant degradation rate of lignin was 45.96%. Also, Fourier transform infrared spectroscopy (FTIR) was employed to changes in functional groups arising from lignin degradation. The results showed that the relative intensity of spectral peaks associated with lignin (1099cm-1and 1057-1038 cm-1) was weakened, whereas the relative intensity of spectral peaks associated with benzene (1643-1608 cm-1 and 1510-1508 cm-1) was enhanced. These results suggest the degradation of lignin into small molecules or lignin monomers. Moreover, the contents of benzene and cyclic compounds in lignin were decreased according to comparisons with the changes of other spectral peaks.

Key words: soybean straw, Phanerochaete chrysosporium Burdsall, lignin, FTIR

中图分类号:

TS202.3

王丽婷，徐伟，张玉强. 黄孢原毛平革菌对大豆秸秆木质素降解的FTIR表征[J]. 食品科学, 2011, 32(19): 137-140.

WANG Li-ting，XU Wei，ZHANG Yu-qiang. FTIR Characterization of Lignin Degradation Products from Soybean Straw Fermented by Phanerochaete chrysosporium Burdsall[J]. FOOD SCIENCE, 2011, 32(19): 137-140.

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黄孢原毛平革菌对大豆秸秆木质素降解的FTIR表征

FTIR Characterization of Lignin Degradation Products from Soybean Straw Fermented by Phanerochaete chrysosporium Burdsall

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