食品科学 ›› 2011, Vol. 32 ›› Issue (19): 244-247.doi: 10.7506/spkx1002-6630-201119055

• 营养卫生 • 上一篇    下一篇

反式脂肪酸对血管内皮细胞损伤的影响

于化泓,梁忠爽,李湘梅,邱 斌,刘 蓉,李 静,邓泽元*   

  1. 南昌大学食品科学与技术国家重点实验室
  • 发布日期:2011-10-12
  • 基金资助:
    国家自然科学基金项目(30972482);江西省学术带头人计划项目(2008DD00900);教育部博士点基金项目(20070403002)

Effects of trans-Fatty Acids on Damage of Human Umbilical Vein Endothelial Cells

YU Hua-hong,LIANG Zhong-shuang,LI Xiang-mei,QIU Bin,LIU Rong,LI Jing,DENG Ze-yuan*   

  1. (State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China)
  • Published:2011-10-12

摘要: 探讨不同反式脂肪酸促动脉粥样硬化(AS)的共同机制。通过采用反油酸和反亚油酸作用于人脐静脉内皮细胞,对其存活率、一氧化氮合酶(NOS)活性、一氧化氮(NO)生成量以及乳酸脱氢酶(LDH)渗出率的影响,比较不同反式脂肪酸对血管内皮细胞损伤的影响。结果表明:反式脂肪酸能抑制细胞增殖,可通过降低NOS活性影响NO分泌,能增加细胞LDH渗出率,从而影响细胞氧化应激水平,降低血管舒张能力。此外,实验发现反亚油酸对细胞损伤能力较反油酸强。

关键词: 反油酸, 反亚油酸, 人脐静脉内皮细胞, 损伤

Abstract: The effects of trans-linoleic acid and trans-oleic acid on survival rate, nitric oxide synthase (NOS) activity, nitric oxide (NO) production and lactate dehydrogenase (LDH) leakage of human umbilical vein endothelial cells was studied to explore the mechanisms by which trans-fatty acids promote atherosclerosis. The results showed that both trans-fatty acids could inhibit cell proliferation through reducing NOS activity and NO secretion, increasing LDH leakage, and decreasing the vasodilation capability, thus affecting the cell oxidative stress level. Moreover, trans-linoleic acid revealed stronger cell damage than trans-oleic acid.

Key words: trans-linoleic acid, trans-oleic acid, human umbilical vein endothelial cells, damage

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