食品科学 ›› 2011, Vol. 32 ›› Issue (21): 37-40.doi: 10.7506/spkx1002-6630-201121009

• 基础研究 • 上一篇    下一篇

褐蘑菇水溶性多糖分级组分抗氧化性能

李芳亮1,高 杨2,刘 莹1,王 锐1   

  1. 1.辽宁工程技术大学理学院 2.辽宁省阜新市科技局
  • 出版日期:2011-11-15 发布日期:2011-11-11
  • 基金资助:
    辽宁工程技术大学优秀青年科学研究基金项目(07-127)

Antioxidant Activity of Water-soluble Polysaccharide Fractions from Portabella Mushroom Agaricus bisporus

LI Fang-liang1,GAO Yang2,LIU Ying1,WANG Rui1   

  1. (1. College of Science, Liaoning Technical University, Fuxin 123000, China; 2. Fuxin Science and Technology Bureau, Fuxin 123000, China)
  • Online:2011-11-15 Published:2011-11-11

摘要: 采用水提醇沉法提取并精制褐蘑菇多糖(WPPA),用体积分数60%、70%和80%乙醇分级醇沉分离得3种粗多糖WPPA60、WPPA70和WPPA80;通过还原力、清除超氧阴离子自由基(O2- ·)、清除羟自由基( ·OH)和抑制H2O2诱导红细胞氧化溶血实验评价3种褐蘑菇多糖体外抗氧化能力。结果表明:WPPA60、WPPA70和WPPA80具有较强的还原能力,对O2- ·和 ·OH具有较强的清除作用;对H2O2诱导红细胞氧化溶血及MDA生成有很强的抑制作用;抗氧化能力从强到弱依次是WPPA70>WPPA80>WPPA60。

关键词: 还原力, 褐蘑菇, 多糖, 抗氧化

Abstract: Water extraction followed by fractional ethanol precipitation was used to purify water-soluble polysaccharides from portabella mushroom Agaricus bisporus (WPPA). The polysaccharide fractions WPPA60, WPPA70 and WPPA80 were obtained using three ethanol concentrations: 60%, 70% and 80%. Their antioxidant activity in vitro was assessed in terms of reducing power, scavenging activity against superoxide anion hydroxyl free radicals and inhibitory activity against H2O2-induced erythrocyte hemolysis. The results showed that WPPA60, WPPA70 and WPPA80 revealed strong reducing power, scavenging capacity against superoxide anion and hydroxyl free radicals and inhibitory effect on erythrocyte hemolysis and MDA generation. The polysaccharide fractions were ranked in decreasing order of antioxidant activity as follows: WPPA70, WPPA80 and WPPA60.

Key words: reducing power, portabella mushroom Agaricus bisporus, polysaccharide, antioxidation

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