食品科学 ›› 2011, Vol. 32 ›› Issue (21): 52-55.doi: 10.7506/spkx1002-6630-201121012

• 基础研究 • 上一篇    下一篇

蜂王浆冻干粉体外抗氧化作用

高 慧,程 妮,贾 琪,王毕妮,邓建军,曹 炜*   

  1. 西北大学化工学院食品科学与工程系
  • 出版日期:2011-11-15 发布日期:2011-11-11
  • 基金资助:
    陕西省科技计划项目(2010K01-22);陕西省教育厅科研计划项目(11JK0623)

in vitro Antioxidant Activity of Lyophilized Rape Royal Jelly

GAO Hui,CHENG Ni,JIA Qi,WANG Bi-ni,DENG Jian-jun,CAO Wei*   

  1. (Department of Food Science and Engineering, School of Chemical Engineering, Northwest University, Xi, an 710069, China)
  • Online:2011-11-15 Published:2011-11-11

摘要: 对青海、陕西、安徽和浙江产油菜蜂王浆的冻干粉中水溶性蛋白、总酚和10-羟基-2-葵烯酸(10-HDA)含量进行测定,并通过DPPH法和FRAP法评价蜂王浆冻干粉的抗氧化活性。结果表明:不同产地油菜蜂王浆的冻干粉中总酚含量无显著性差异,但青海产油菜蜂王浆的冻干粉中水溶性蛋白含量最高(190.32mg/g),10-HDA含量最高的为陕西产油菜蜂王浆的冻干粉(4.28%);不同产地油菜蜂王浆的冻干粉均具有良好的DPPH自由基清除能力和总抗氧化能力,且样品的总抗氧化能力与其所含有的水溶性蛋白(r=0.88)和总酚含量(r=0.95)间呈显著正相关。

关键词: 蜂王浆, 水溶性蛋白, 10-HDA, 抗氧化作用

Abstract: Water-soluble protein, total phenol and 10-HDA in lyophilized samples of rape royal jelly from different regions: Qinghai, Shaanxi, Anhui and Zhejiang were analyzed in this study. The antioxidant activities of water-soluble extract from these samples were determined using DPPH radical scavenging and FRAP assays. The results showed that there were no significant differences in total phenol content among royal jelly samples from the different regions above. Royal jelly samples collected from Qinghai and Shaanxi had the highest water-soluble protein and 10-HDA contents, reaching 190.32 mg/g and 4.28%, respectively. All the samples investigated had powerful DPPH radical scavenging activity and total antioxidant capacity. Strong correlations were observed between total antioxidant capacity and water-soluble protein content (r=0.88) or total phenol content (r=0.95).

Key words: lyophilized royal jelly, water-soluble proteins, 10-HAD, antioxidant activity

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