食品科学 ›› 2011, Vol. 32 ›› Issue (21): 66-70.doi: 10.7506/spkx1002-6630-201121015

• 基础研究 • 上一篇    下一篇

蜂蜜中氨基酸含量对抗氧化能力的影响

董 蕊,郑毅男*   

  1. 吉林农业大学中药材学院
  • 收稿日期:2018-06-20 修回日期:2018-06-20 出版日期:2011-11-15 发布日期:2011-11-11

Effects of Amino Acid Content on Antioxidant Activity of Honey from Different Sources and Geographic Origins

DONG Rui,ZHENG Yi-nan*   

  1. (College of Traditional Chinese Medicine, Jilin Agricultural University, Changchun 130118, China)
  • Received:2018-06-20 Revised:2018-06-20 Online:2011-11-15 Published:2011-11-11

摘要: 以14种中国单花蜜为原料,比较所含有的17种游离氨基酸含量差异,确定样品中主要以脯氨酸为主,占游离氨基酸总量65.27%,平均含游离氨基酸349.829mg/kg。评估14种单花蜜体外抗氧化活性,得到单花蜜对亚硝酸盐抑制能力的EC50值从(0.55±0.021)g/mL到(0.25±0.034)g/mL之间变化,广西的龙眼蜜抑制能力最强;14种单花蜜中脯氨酸、游离氨基酸含量差异分别与对亚硝酸盐抑制能力的相关系数R=0.7513和R=0.8051。说明蜂蜜中氨基酸类物质影响抗氧化活性,其含量与对亚硝酸盐的抑制能力呈现正相关关系,蜂蜜作为潜在的功能性食品更应值得关注。

关键词: 蜂蜜, 氨基酸, 抗氧化, 功能性食品

Abstract: Significant differences were found in 17 free amino acids among 14 honey samples from different regions of China and different flowers. The result showed that proline was the major free amino acid and constituted over 65.27% of total free amino acids, averaging at about 349.829 mg/kg in the honey samples. The antioxidant ability of honeys was evaluated by NO2- · radical scavenging assay. The results obtained showed that all the honey samples had antioxidant activity, with an EC50 ranging from (0.55±0.021) to (0.25±0.034) g/mL. Among these samples, longan honey from Guangxi had the highest antioxidant ability. Correlation analysis revealed that the antioxidant ability of honeys was correlated with proline content and the contents of 17 total free amino acid, with R=0.7513 and R=0.8051 respectively. Conclusion: Free amino acids in honeys are positively correlated with NO2- · radical scavenging activity. As a known functional food, honey may have more health benefits than previously reported and more attention should be paid to it for its full utilization.

Key words: honey, free amino acids, antioxidant ability, functional foods

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