食品科学 ›› 2011, Vol. 32 ›› Issue (21): 210-213.doi: 10.7506/spkx1002-6630-201121043

• 生物工程 • 上一篇    下一篇

一株高产黑色素暗盘菌菌株的选育

胡雪芹1,钱梅双2,朱 立2,郭赓艺2,叶 明2,*   

  1. 1.合肥工业大学化工学院 2.合肥工业大学生物与食品工程学院
  • 出版日期:2011-11-15 发布日期:2011-11-11
  • 基金资助:
    安徽省自然科学基金项目(090413086)

Breeding of a High Melanin-producing Plectania Strain

HU Xue-qin1,QIAN Mei-shuang2,ZHU Li2,GUO Geng-yi2,YE Ming2,*   

  1. (1. School of Chemical Engineering, Hefei University of Technology, Hefei 230009, China; 2. College of Biotechnology and Food Engineering, Hefei University of Technology, Hefei 230009, China)
  • Online:2011-11-15 Published:2011-11-11

摘要: 研究黑色素暗盘菌Plectania YM421原生质体制备的最佳条件,并对该原生质体进行紫外诱变以获得高产黑色素菌株。结果表明:在质量浓度为2.5g/100mL蜗牛酶,33℃酶解3h的条件下,Plectania YM421原生质体形成率最高;在距离20W紫外灯下30cm处,紫外诱变时间在15~25s之间时,获得突变株YM421-2#黑色素的产量较出发菌株提高了10.03%,生物量提高了11.33%。该突变株可以作为黑色素工业生产的候选菌株。

关键词: 暗盘菌, 原生质体, 紫外诱变, 黑色素, 生物量

Abstract: In order to breed a Plectania strain with higher melanin-producing ability, the preparation and UV mutagenization of protoplasts from Plectania YM421 mycelium was optimized. Results indicated that maximum rate of protoplast formation was observed after 3 h hydrolysis of helicase added at a level of 2.5 g/100 mL at 33 ℃. After exposure to UV radiation for 15-25 s at the distance of 30 cm, mutant strain YM421-2# was obtained, which exhibited respective increases of melanin yield by 10.03% and biomass by 11.33% when compared with the start strain. This mutant strain can be used for industrial production of melanin as a candidate.

Key words: Plectania, protoplast, UV mutagenesis, melanin, biomass

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