食品科学 ›› 2018, Vol. 39 ›› Issue (13): 184-189.doi: 10.7506/spkx1002-6630-201813027

• 营养卫生 • 上一篇    下一篇

不同来源乳铁蛋白及乳铁蛋白素对小鼠脾淋巴细胞增殖影响的比较

皮冰冰1,赵 晓1,吕萍萍2,彭钰凯1,潘 琳1,许晓曦1,*   

  1. 1.东北农业大学食品学院,黑龙江 哈尔滨 150030;2.绥芬河出入境检验检疫局,黑龙江 绥芬河 157300
  • 出版日期:2018-07-15 发布日期:2018-07-09
  • 基金资助:
    “十二五”国家科技支撑计划项目(2013BAD18B00)

Comparative Study on the Effects of Lactoferrin and Lactoferricin from Different Sources on Proliferation of Mouse Spleen Lymphocytes

PI Bingbing1, ZHAO Xiao1, Lü Pingping2, PENG Yukai1, PAN Lin1, XU Xiaoxi1,*   

  1. 1. Food Science College, Northeast Agricultural University, Harbin 150030, China; 2. Suifenhe Entry-Exit Inspection and Quarantine Bureau, Suifenhe 157300, China
  • Online:2018-07-15 Published:2018-07-09

摘要: 以牛乳清乳铁蛋白(bovine whey lactoferrin,LFW)、牛初乳乳铁蛋白(bovine colostrum lactoferrin, LFC)、人乳铁蛋白(human lactoferrin,LFH)、牛乳铁蛋白素(bovine lactoferricin,LfcinB)和人乳铁蛋白 素(human lactoferricin,LfcinH)为研究对象,研究其对小鼠脾淋巴细胞增殖的影响并比较不同来源乳铁蛋白 (lactoferrin,LF)及乳铁蛋白素(lactoferricin,Lfcin)作用效果的差异性。采用CCK-8法检测不同来源的LF与 Lfcin作用小鼠脾淋巴细胞24、48、72 h后,及其与丝裂原共同作用对小鼠T、B淋巴细胞增殖作用的影响。结果显 示:LF与Lfcin均在48 h时对小鼠脾淋巴细胞增殖的促进效果最好,且在一定质量浓度范围内促进效果随质量浓度升 高而加强;LF与Lfcin均在一定质量浓度范围内提高刀豆蛋白A诱导的T淋巴细胞的增殖和脂多糖诱导的B淋巴细胞 的增殖。结果表明:两种牛乳铁蛋白(bovine lactoferrin,LFB)及LfcinB可替代LFH应用于婴幼儿配方产品中以提 高婴幼儿免疫能力,LFB推荐添加质量浓度为0.50~2.00 mg/mL,Lfcin推荐添加质量浓度为75~125 μg/mL。

关键词: 乳铁蛋白, 乳铁蛋白素, 脾淋巴细胞, 增殖

Abstract: The effects of lactoferrin from bovine whey (LFW), lactoferrin from bovine colostrum (LFC), human lactoferrin (LFH), human lactoferricin (LfcinH) and bovine lactoferricin (LfcinB) on the proliferation of spleen lymphocytes from mice were studied, and different lactoferrin (LF) and lactoferricin (Lfcin) were compared. The cell counting kit-8 assay was used to measure cell proliferation after 24, 48 and 72 h of culture. Moreover, the effects of different LF and Lfcin combined with mitogen on the proliferation of mouse T and B lymphocytes were also evaluated. The results showed that both LF and Lfcin had the most significant effect in promoting the proliferation of spleen lymphocytes at 48 h, which was concentration-dependent in a certain range. Both LF and LFcin enhanced T lymphocyte proliferation induced by concanavalin A and B lymphocyte proliferation induced by lipopolysaccharide in a certain concentration range. These findings indicated that the two types of bovine lactoferrins (LFB) as well as Lfcin can substitute LFH in infant formula products to enhance immune function in infants and young children, and the recommended concentration for LFB and Lfcin was 0.50–2.00 mg/mL and 75–125 μg/mL, respectively.

Key words: lactoferrin, lactoferricin, spleen lymphocyte, proliferation

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