蓝靛果花色苷对高脂血症大鼠肝脏LDLR、ABCG1及ABCA1基因表达的影响

doi:10.7506/spkx1002-6630-201813030

食品科学 ›› 2018, Vol. 39 ›› Issue (13): 205-210.doi: 10.7506/spkx1002-6630-201813030

蓝靛果花色苷对高脂血症大鼠肝脏LDLR、ABCG1及ABCA1基因表达的影响

于伟1,2，张桂芳2，宋雪建3，甄井龙3，迟晓星3，王振宇4,*

1.东北林业大学林学院，黑龙江哈尔滨 150040；2.黑龙江八一农垦大学国家杂粮工程技术研究中心，黑龙江大庆 163319；3.黑龙江八一农垦大学食品学院，黑龙江大庆 163319；4.哈尔滨工业大学食品科学与工程系，黑龙江哈尔滨 150001

出版日期:2018-07-15 发布日期:2018-07-09
基金资助:
黑龙江省教育厅科学技术研究项目（125416000）；国家自然科学基金面上项目（81673170）

Effect of Lonicera caerulea Anthocyanins on the Expression of LDLR, ABCG1 and ABCA1 Genes in Livers of Hyperlipidemic Rats

YU Wei1,2, ZHANG Guifang2, SONG Xuejian3, ZHEN Jinglong3, CHI Xiaoxing3, WANG Zhenyu4,*

1. School of Forestry, Northeast Forestry University, Harbin 150040, China; 2. National Coarse Cereals Engineering Research Center, Heilongjiang Bayi Agricultural University, Daqing 163319, China; 3. College of Food Science, Heilongjiang Bayi Agricultural University, Daqing 163319, China; 4. Department of Food Science and Engineering, Harbin Institute of Technology, Harbin 150001, China

Online:2018-07-15 Published:2018-07-09

摘要/Abstract

摘要： 目的：研究蓝靛果花色苷对高脂血症大鼠肝脏低密度脂蛋白受体（low density lipoprotein receptor， LDLR）、三磷酸腺苷结合盒转运体G1（ATP-binding cassette transporter G1，ABCG1）及ABCA1基因表达的影响。方法：选择2 月龄雄性Wistar大鼠60 只，将大鼠随机分为6 组，分别为基础饲料对照组（ND，1.2 g/（kg·d mb）生理盐水灌胃）、高脂模型对照组（HFD，1.2 g/（kg·d mb）生理盐水灌胃）、阳性对照组（10 mg/（kg·d mb）辛伐他汀片灌胃），蓝靛果花色苷低、中、高剂量组（HFD＋L、HFD＋M、HFD＋H，分别给予4.0、40.0、 120.0 mg/（kg·d mb）的花色苷灌胃），持续28 d。实验结束后，测定血清总胆固醇（total cholesterol，TC）、甘油三酯（triglyceride，TG）、高密度脂蛋白胆固醇（high density lipoprotein cholesterol，HDL-C）、低密度脂蛋白胆固醇（low density lipoprotein cholesterol，LDL-C）、载脂蛋白A（apolipoprotein A，Apo-A）及载脂蛋白B（Apo-B）等血脂指标水平。取大鼠肝脏，利用实时荧光定量聚合酶链式反应测定大鼠肝脏组织中 LDLR、ABCG1、ABCA1 mRNA表达量，Western blot检测LDLR蛋白表达水平。结果：蓝靛果花色苷干预后，与HFD组相比，花色苷均能不同程度地降低高血脂大鼠血清中TC、TG、LDL-C、Apo-B的含量（P＜0.05或 P＜0.01），显著升高HDL-C及Apo-A的含量（P＜0.05或P＜0.01）。花色苷各剂量组LDLR蛋白和mRNA水平均增高，与HFD组比较差异有显著性（P＜0.05），ABCA1 mRNA和ABCG1 mRNA的表达水平也高于HFD组，尤其是花色苷中、高剂量组差异明显（P＜0.05）。结论：40.0 mg/（kg·d mb）蓝靛果花色苷具有明显的调节血脂作用，其作用机制可能是通过上调肝脏LDLR和ABC家族基因的表达，进而调节胆固醇逆转运过程。

关键词: 蓝靛果花色苷, 高脂大鼠, 低密度脂蛋白受体, 三磷酸腺苷结合盒转运体G1, 三磷酸腺苷结合盒转运体A1

Abstract: Objective: To investigate the effect of anthocyanins from the fruits of Lonicera caerulea (LCA) on the gene expression of low density lipoprotein receptor (LDLR), ATP-binding cassette transporter G1 (ABCG1), and ATP-binding cassette transporter A1 (ABCA1) in hyperlipidemic rat liver. Methods: A total of 60 male Wistar rats aged two months were randomly divided into 6 groups: normal diet control (ND, intragastrically administered with 1.2 g/(kg·d mb) of physiological saline), high-fat diet control (HFD, similarly administered with 1.2 g/(kg·d mb) of physiological saline), positive control (intragastrically administered with 10 mg/(kg·d mb) of simvastatin tablets), and low-, middle- and high-dose LCA (at 4.0, 40.0 and 120.0 mg/(kg·d mb) groups. The administration lasted for 28 days. At the end of this period, the levels of serum total cholesterol (TC) and triglyceride (TG), HDL cholesterol (HDL-C), low density lipoprotein cholesterol (LDL-C), apolipoprotein A (Apo-A) and apolipoprotein B (Apo-B) were determined. The mRNA expression levels of LDLR, ABCA1 and ABCG1 were detected by real-time polymerase chain reaction (RT-PCR) and the protein level of LDLR was evaluated by Western blot analysis. Results: Compared with the hyperlipemia model group, LCA could reduce the serum levels of TC, TG, LDL-C and Apo-B and increase the levels of HDL-C and Apo-A (P < 0.05 or P < 0.01). The expression of LDLR protein and mRNA in all dose groups increased significantly (P < 0.05), and the expression levels of ABCA1 and ABCG1 mRNA similarly rose; in particular, a significant increase was observed in the middle- and high-dose groups (P < 0.05). Conclusion: 40.0 mg/(kg·d mb) Lonicera caerulea anthocyanins have a significant role in regulating blood lipids by up-regulating the expression of LDLR and ABC family genes in the liver and consequently regulating reverse cholesterol transportation in hyperlipidemic rats.

Key words: Lonicera caerulea anthocyan, hyperlipidemic rats, low density lipoprotein receptor, ATP-binding cassette transponter G1, ATP-binding cassette transporter A1

中图分类号:

TS201.4

于伟，张桂芳，宋雪建，甄井龙，迟晓星，王振宇. 蓝靛果花色苷对高脂血症大鼠肝脏LDLR、ABCG1及ABCA1基因表达的影响[J]. 食品科学, 2018, 39(13): 205-210.

YU Wei, ZHANG Guifang, SONG Xuejian, ZHEN Jinglong, CHI Xiaoxing, WANG Zhenyu. Effect of Lonicera caerulea Anthocyanins on the Expression of LDLR, ABCG1 and ABCA1 Genes in Livers of Hyperlipidemic Rats[J]. FOOD SCIENCE, 2018, 39(13): 205-210.

[1]	于伟，张桂芳，甄井龙，宋雪建，迟晓星，王振宇. 蓝靛果花色苷对高脂血症大鼠肝脏内与胆固醇代谢相关基因的作用[J]. 食品科学, 2018, 39(17): 171-176.
[2]	盛倩，雷秋霞，曹顶国，王存芳. 鸡卵黄前体物和受体及其对蛋品品质的影响[J]. 食品科学, 2012, 33(9): 260-263.

蓝靛果花色苷对高脂血症大鼠肝脏LDLR、ABCG1及ABCA1基因表达的影响

Effect of Lonicera caerulea Anthocyanins on the Expression of LDLR, ABCG1 and ABCA1 Genes in Livers of Hyperlipidemic Rats

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