食品科学 ›› 2011, Vol. 32 ›› Issue (23): 58-63.doi: 10.7506/spkx1002-6630-201123010

• 基础研究 • 上一篇    下一篇

白背三七多糖的分离纯化

刘微微,刘 旭,曹学丽*   

  1. 北京工商大学食品学院,食品添加剂与配料北京高校工程研究中心
  • 出版日期:2011-12-15 发布日期:2011-12-21

Separation and Purification of Polysaccharides from Leaves of Gynura davaricata (L.) DC.

LIU Wei-wei,LIU Xu,CAO Xue-li*   

  1. (Beijing Higher Institution Engineering Research Center of Food Additives and Ingredients, School of Food and Chemical Engineering, Beijing Technology and Business University, Beijing 100048, China)
  • Online:2011-12-15 Published:2011-12-21

摘要: 采用D315弱碱性阴离子交换树脂和Sephadex G-25凝胶柱层析,从白背三七叶粗多糖中分离纯化得到中性多糖GDPs-1、GDPs-2及酸性多糖GDPs-3 3个多糖组分。经高效凝胶色谱测定,重均分子质量分别为:1.06×104、4.17×103D和3.74×103D;纯度分别为94.08%、92.3%和99.8%。红外光谱证明3种多糖均含有多糖类特征吸收峰,初步推测GDPs-1中α和β构型并存,GDPs-2和GDPs-3为α构型;核磁共振结果进一步证实3种多糖中均只含有α构型。

关键词: 白背三七, 多糖, 分离纯化, 红外光谱, 构型, 核磁共振

Abstract: Two neutral polysaccharides (GDPs-1 and GDPs-2) and one acidic polysaccharide (GDPs-3) were separated and purified from water-soluble crude polysaccharide from the leaves of Gynura davaricata (L.) DC. by D315 resin column and subsequent Sephadex G-25 gel column chromatography. GPC analysis showed that the average molecular weights of GDPs-1, GDPs-2 and GDPs-3 were 1.06 × 104, 4.17 × 103 D, and 3.74 × 103 D with a purity of 94.08%, 92.3% and 99.8%, respectively. Infrared spectra preliminarily confirmed that the purified fractions were polysaccharides. GDPs-1 was characterized as α- and β-configuration, and GDPs-2 and GDPs-3 asα-configuration. Nuclear magnetic resonance (1H-NMR) results further proved that the polysaccharides contained only α-configuration. In conclusion, all these investigations can provide reference data for further studies on structural characterization and bioactivity of Gynura davaricata polysaccharides.

Key words: Gynura davaricata (L.) DC., polysaccharide, separation and purification, infrared spectrum, configuration, nuclear magnetic resonance

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