食品科学 ›› 2011, Vol. 32 ›› Issue (23): 172-175.doi: 10.7506/spkx1002-6630-201123034

• 生物工程 • 上一篇    下一篇

大豆发芽过程中多肽富集条件优化

饶青青1,余晓红1,2,唐军涛1,韩永斌1,顾振新1,*   

  1. 1.南京农业大学食品科技学院 2.盐城工学院化学与生物工程学院
  • 出版日期:2011-12-15 发布日期:2011-12-21
  • 基金资助:
    江苏省科技支撑计划项目(BE2010386)

Optimization of Polypeptide Accumulation Conditions during Soybean Germination

RAO Qing-qing1,YU Xiao-hong1,2,TANG Jun-tao1,HAN Yong-bin1,GU Zhen-xin1,*   

  1. (1. College of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, China; 2. College of Chemistry and Biological Engineering, Yancheng Institute of Technology, Yancheng 224003, China)
  • Online:2011-12-15 Published:2011-12-21

摘要: 目的:研究培养液pH值、培养时间及培养温度对大豆发芽过程中多肽富集的影响。方法:通过响应面试验法对培养条件进行优化,确定发芽大豆多肽最优富集工艺。结果:培养液pH值、培养时间及培养温度对发芽大豆中的多肽含量变化影响显著。结论:大豆最适培养条件为:以pH5.2的柠檬酸-柠檬酸钠缓冲液(5mmol/L)作为培养液、培养温度29.0℃、培养时间6d,在此条件下,发芽大豆多肽含量为159.38mg/g,是原料大豆中的3.83倍。

关键词: 大豆, 发芽, 多肽, 富集

Abstract: Objective: To explore the effect of pH, culture time and culture temperature on polypeptide accumulation in soybean during germination. Methods: Culture conditions optimization for maximizing polypeptide accumulation was performed using response surface methodology based on Box-Behnken experimental design. Results: All the investigated conditions were found to significantly affect the content of polypeptides during the germination process of soybean. Conclusion: The optimal conditions for the accumulation of polypeptides in germinated soybean were achieved after 6 days of culture at 29.0 ℃ in 5 mmol/L, pH 5.2 CBS buffer. The optimized culture resulted in a polypeptide content of 159.38 mg/g soybean, revealing a 3.83-fold increase in compared with non-germinated soybeans.

Key words: soybean, germination, polypeptide, accumulation

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