蚕蛹蛋白酶解产物体外抗氧化和降血压活性筛选及响应面工艺优化

doi:10.7506/spkx1002-6630-201123037

食品科学 ›› 2011, Vol. 32 ›› Issue (23): 186-191.doi: 10.7506/spkx1002-6630-201123037

蚕蛹蛋白酶解产物体外抗氧化和降血压活性筛选及响应面工艺优化

赵钟兴1，廖丹葵1，孙建华1，黄科林2，孙果宋2，秦伟嘉1，吴志洪2，童张法1,*

1.广西大学化学化工学院，广西石化资源加工及过程强化技术重点实验室 2.广西化工研究院

出版日期:2011-12-15 发布日期:2011-12-21
基金资助:
广西自然科学基金项目(2010GXNSFA013058)；广西研究生教育创新计划资助项目(105930901005)；广西大学科研基金资助项目(XGL090021)

Screening for Optimal Enzyme for Preparation of Silkworm Pupae Protein Hydrolysate with in vitro Antixodiant and Antihypertensive Activities and Process Optimization by Response Surface Methodology

ZHAO Zhong-xing1，LIAO Dan-kui1，SUN Jian-hua1，HUANG Ke-lin2，SUN Guo-song2，QIN Wei-jia1， WU Zhi-hong2，TONG Zhang-fa1,*

(1. Guangxi Key Laboratory of Petrochemical Resources Processing and Process Intensification Technology, School of Chemistry and Chemical Engineering, Guangxi University, Nanning 530004, China； 2. Guangxi Research Institute of Chemical Industry, Nanning 530004, China)

Online:2011-12-15 Published:2011-12-21

摘要/Abstract

摘要： 以蚕蛹蛋白为原料，使用碱性蛋白酶、木瓜蛋白酶、胰蛋白酶、菠萝蛋白酶对其进行酶解，采用1,1-一苯基-2-三硝基苯肼自由基(DPPH自由基)法、超氧阴离子自由基(O2－ ·)法、羟自由基( ·OH)法和血压紧张素转化酶(ACE)法对酶解产物进行抗氧化和降血压活性分析，筛选同时具有较强抗氧化和降血压活性的酶解产物，并确定获得较强活性时的水解度，再通过响应面对该水解度下酶解工艺条件进行优化。结果表明：碱性蛋白酶在水解度为20%左右时获得的水解产物抗氧化和降血压效果较好，并由响应面分析结果表明酶解温度和加酶量之间存在显著的交互作用，同时得到最佳酶解工艺条件为：酶解温度51℃，pH值为9.05，加酶量为0.38%，酶解时间3h，在此条件下，水解度为20.53%，再通过实验验证其误差值小于5%，说明采用响应面试验设计方法对蚕蛹蛋白酶解体系进行优化具有较好的可靠性。此时酶解产物的DPPH自由基抑制率为51.2%，ACE抑制活性为50.3%。

关键词: 蚕蛹蛋白, 抗氧化肽, 降血压肽, 响应面, 水解度

Abstract: In the present study, silkworm pupae protein hydrolysates at different degree of hydrolysis were prepared with alcalase, papain, trypsin, and bromelain, respectively and their scavenging activity against DPPH, superoxide anion and hydroxyl free radicals and antihypertensive activity were comparatively determined, with the result that hydrolyzing silkworm pupae protein by alcalase to a degree of hydrolysis (DH) of 20% yielded a hydrolysate with better antixodiant and antihypertensive activities. Three main process conditions for the hydrolysis of silkworm pupae protein by alcalase, including temperature, pH and enzyme dosage were further optimized using response surface methodology. The results of response surface analysis indicated that temperature and enzyme dosage had a significant interactive effect on the hydrolysis of silkworm pupae protein and that the optimal hydrolysis conditions for achieving 20% DH and minimizing costs were 51.0 ℃, pH 9.05 and 0.38% enzyme/substrate ratio. Under these conditions, the experimental DH was 20.53%, with an error of less than 5% as compared with the predictive value, and the DPPH radical scavenging rate and ACE inhibitory rate of the obtained hydrolysate were 51.2% and 50.3%, respectively.

Key words: silkworm pupae protein, antioxidant peptide, ACE inhibitory peptide, response surface methodology, degree of hydrolysis

中图分类号:

Q566.3

赵钟兴，廖丹葵，孙建华，黄科林，孙果宋，秦伟嘉，吴志洪，童张法. 蚕蛹蛋白酶解产物体外抗氧化和降血压活性筛选及响应面工艺优化[J]. 食品科学, 2011, 32(23): 186-191.

ZHAOZhong-xing，LIAODan-kui，SUNJian-hua，HUANGKe-lin，SUNGuo-song，QINWei-jia，WUZhi-hong，TONGZhang-fa. Screening for Optimal Enzyme for Preparation of Silkworm Pupae Protein Hydrolysate with in vitro Antixodiant and Antihypertensive Activities and Process Optimization by Response Surface Methodology[J]. FOOD SCIENCE, 2011, 32(23): 186-191.

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蚕蛹蛋白酶解产物体外抗氧化和降血压活性筛选及响应面工艺优化

Screening for Optimal Enzyme for Preparation of Silkworm Pupae Protein Hydrolysate with in vitro Antixodiant and Antihypertensive Activities and Process Optimization by Response Surface Methodology

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