食品科学 ›› 2011, Vol. 32 ›› Issue (23): 192-196.doi: 10.7506/spkx1002-6630-201123038

• 生物工程 • 上一篇    下一篇

多粮浓香型白酒生产中放线菌的多样性

张 超1,2,3,赵 东4,王 涛1,2,3,游 玲2,3,冯瑞章1,2,王 松1,2,3,何建蓉5   

  1. 1.宜宾学院生命科学与食品工程学院 2.发酵资源与应用四川省高校重点实验室 3.宜宾学院发酵工程与食品科学研究所 4.五粮液集团技术中心 5.宜宾红楼梦酒业股份有限公司
  • 出版日期:2011-12-15 发布日期:2011-12-21
  • 基金资助:
    四川省科技厅社会发展科技支撑项目(2008NZ0031;2010NZ0029;2010NZ0091); 四川省科技厅应用基础项目(2009JY0149);四川省教育厅重大培育项目(09ZZ039); 四川省属高校白酒生产技术创新团队建设计划项目;宜宾市科技专项研究基金项目(200805303;2010ZGY016)

Diversity of Actinomycetes in the Brewing Process of Luzhou-flavor Multiple-grains Liquor

ZHANG Chao1,2,3,ZHAO Dong4,WANG Tao1,2,3,YOU Ling2,3,FENG Rui-zhang1,2,WANG Song1,2,3,HE Jian-rong5   

  1. (1. College of Life Science and Food Engineering, Yibin University, Yibin 644000, China;2. Key Laboratory of Fermentation Resource and Application of Institutes of Higher Learning in Sichuan, Yibin 644000, China;3. Fermentation Engineering and Food Science Institute, Yibin University, Yibin 644000, China;4. Technogical Center of Wuliangye Group Co. Ltd., Yibin 644000, China;5. Hongloumeng Distillery Co. Ltd., Yibin 644000, China)
  • Online:2011-12-15 Published:2011-12-21

摘要: 以改良的高氏I号培养基为基质,从多家宜宾多粮浓香型白酒生产企业的窖房空气、曲房空气、窖泥和糟醅分离到123株典型丝状放线菌,并对其进行了基于16S rRNA基因序列的系统发育分析。结果显示;123株典型放线菌分属于Streptomyce、Massilia、Nocardiopsis 3个属,Streptomyce为绝对优势属(121株,占总菌株数的98.4%);Streptomyce属的121株菌与该属内26个种的典型菌株序列相似度≥98%,其中,有45株与S. violascens ISP 5183T序列最接近,分布于4个取样点;在4个取样点中,窖泥的丰富度指数和多样性指数最高,糟醅的优势度指数最高;各样点间放线菌的相似性较低,且都存在特有的放线菌类群。

关键词: 浓香型白酒, 放线菌, 16S rRNA基因序列, 系统发育分析, 多样性

Abstract: A total of 123 typical strains of actinomycetes were isolated from the air in fermentation workshop and Qu workshop, pit mud and fermented grains in several distilleries of Luzhou-flavor multiple-grains using modified Gogan-I medium. Their phylogenetic analysis was carried out based on 16S rRNA gene sequence. The results showed that the 123 strains belonged to 3 genera (Streptomyces, Massilia and Nocardiopsis), with Streptomyces being the dominant one (121 strains, accounting for 98.4% of the total isolated 123 strains). The 16S rRNA sequence similarity of the isolated 121 Streptomyces strains to those of the 26 typical strains published in Genbank was more than 98%, among which 45 strains were most closely related to the typical strains S. violascens ISP 5183T and distributed in 4 sample plots. Among the 4 sample plots, the richness index and diversity index was the highest in pit mud, and the dominance index was the highest in fermented grains. The similarity index among 4 sample plots was low and special species of actinomycetes existed in every plots.

Key words: Luzhou-flavor multiple-grains liquor, actinomycetes, 16S rRNA gene sequence, phylogenetic analysis, diversity

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