食品科学 ›› 2011, Vol. 32 ›› Issue (23): 201-205.doi: 10.7506/spkx1002-6630-201123040

• 生物工程 • 上一篇    下一篇

红酵母产β-胡萝卜素体外转化为VA的研究

刘卉琳1,刘 绍1,2,谢达平3,   

  1. 1.湖南农业大学生物安全与技术学院 2.湖南农业大学食品科技学院 3.湖南农业大学生命科学技术学院
  • 出版日期:2011-12-15 发布日期:2011-12-21

in vitro Conversion of β-Carotene Produced by Rhodotorula into Vitamin A

LIU Hui-lin1,LIU Shao1,2,XIE Da-ping3,*   

  1. (1. College of Bio-Safety Science and Technology, Hunan Agricultural University, Changsha 410128, China; 2. College of Food Science and Technology, Hunan Agricultural University, Changsha 410128, China; 3. College of Bio-Science and Technology, Hunan Agricultural University, Changsha 410128, China)
  • Online:2011-12-15 Published:2011-12-21

摘要: 通过高效液相色谱分析红酵母液态发酵提取液,表明该提取液中含有β-胡萝卜素,体外转化分析表明该β-胡萝卜素可转化为VA。通过对转化条件的优化研究,在β-胡萝卜素质量浓度123mg/L浓缩液中,β-胡萝卜素体外转化VA的最佳转化条件为pH8.0、脱氧胆酸钠3.5mmol/L、d-α-生育酚0.5mmol/L 和Tween-40添加量0.25g/100mL,在该最佳反应条件下,红酵母液态发酵所产β-胡萝卜素在2.58nmol/(mg ·h) β-胡萝卜素-15,15'-单加氧酶作用下37℃水浴振荡反应7h,生成40.1mg/L VA,转化率达到32.6%。

关键词: 红酵母, β-胡萝卜素, β-胡萝卜素-15, 15'-单加氧酶, VA, 条件优化

Abstract: The existence ofβ-carotene in the fermentation broth of Rhodotorula was identified by HPLC, and its in vitro cleavage into retinol also known as vitamin A byβ-carotene 15,15'-monooxygenase from chicken intestinal mucosa was studied. The optimum reaction conditions for converting 123 mg/Lβ-carotene concentrate were determined to be: pH 8.0, 3.5 mmol/L deoxycholate, 0.5 mmol/L d-α-tocopherol and 0.25 g/100 mL Tween-40. Under the optimum conditions, 7 h incubation of 2.58 nmol/(mg ·h )β-carotene 15,15'-monooxygenase in 37 ℃ water bath with shaking produced 40.1 mg/L retinol, with a conversion yield of 32.6%.

Key words: Rhodotorula, β-carotene, β-carotene-15,15O`-monooxygenase, retinol, reaction conditions optimization

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