丰年虫蛋白质分类提取和工艺优化及抗氧化性

doi:10.7506/spkx1002-6630-201124006

食品科学 ›› 2011, Vol. 32 ›› Issue (24): 26-30.doi: 10.7506/spkx1002-6630-201124006

丰年虫蛋白质分类提取和工艺优化及抗氧化性

聂路，张建新*，彭鑫华，王欣，黄晓燕

西北农林科技大学食品科学与工程学院

出版日期:2011-12-25 发布日期:2011-12-29
基金资助:
国家级大学生创新项目(G2009A18)

Extraction Optimization and Antioxidant Activity of Water-Soluble Protein from Chirocephalus diaphanous

NIE Lu，ZHANG Jian-xin*，PENG Xin-hua，WANG Xin，HUANG Xiao-yan

(College of Food Science and Engineering, Northwest A&F University, Yangling 712100, China)

Online:2011-12-25 Published:2011-12-29

摘要/Abstract

摘要： 目的：对丰年虫蛋白质进行分类提取和抗氧化活性比较，并对抗氧化活性最强的蛋白质进行提取工艺优化。方法：从丰年虫脱脂粉中分离出水溶性、碱溶性、酸溶性和醇溶性蛋白，并对其进行体外抗氧化活性研究，选择一种抗氧化活性最强的蛋白质，利用中心组合试验设计对其提取工艺进行优化。结果：丰年虫脱脂粉中最主要的蛋白是水溶性蛋白和碱溶性蛋白，其次是醇溶性蛋白，最少的是酸溶性蛋白，该4类分别占脱脂粉的25.03%、26.98%、1.83%、0.53%；通过体外抗氧化研究发现水溶性蛋白抗氧化活性最强；得到提取水溶性蛋白的最优工艺条件是料液比1:7.5、提取温度49.8℃、提取时间2.8h，预测值和实测值的偏差为0.88%。结论：丰年虫水溶性蛋白的抗氧化活性最强，优化的提取工艺条件具有可行性。

关键词: 丰年虫蛋白质, 分类提取, 抗氧化性, 工艺优化

Abstract: Objective: To compare the antioxidant activity of different protein fractions from Chirocephalus diaphanous and optimize the extraction of the fraction with the strongest antioxidant activity. Methods: The residue left after water-soluble protein extraction of Chirocephalus diaphanous was extracted with 0.1 mol/L aqueous NaOH solution, 0.1 mol/L aqueous acetic acid solution and 95% ethanol, respectively. The antioxidant activity of the water-soluble, acid-soluble protein, alkaline-soluble protein and alcohol-soluble proteins obtained was comparatively assessed based on their DPPH, hydroxyl and superoxide anion radical activities and total reducing power to identify the protein fraction with the most powerful antioxidant activity among them. Moreover, response surface methodology based on central composite design was used to optimize the extraction of the most antioxidant protein fraction from Chirocephalus diaphanous. Results: The contents of water-soluble, alkaline-soluble, alcohol-soluble and acid-soluble proteins were 25.03%, 26.98%, 1.83% and 0.53%, respectively. Water-soluble protein from Chirocephalus diaphanous had stronger antioxidant activity than other three proteins. The best results for the extraction of eater-soluble protein from Chirocephalus diaphanous were achieved after 2.8 h extraction at 49.8 ℃ and a material-to-liquid ratio of 1:7.5. The error between the predicted and experimental extraction yields was 0.88%. Conclusions: Water-soluble protein has the highest antioxidant activity and its optimal extraction process is feasible.

Key words: Chirocephalus diaphanous, protein, sequential extraction, antioxidant activity, process optimization

中图分类号:

TS254.4

聂路，张建新，彭鑫华，王欣，黄晓燕. 丰年虫蛋白质分类提取和工艺优化及抗氧化性[J]. 食品科学, 2011, 32(24): 26-30.

NIE Lu，ZHANG Jian-xin，PENG Xin-hua，WANG Xin，HUANG Xiao-yan. Extraction Optimization and Antioxidant Activity of Water-Soluble Protein from Chirocephalus diaphanous[J]. FOOD SCIENCE, 2011, 32(24): 26-30.

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丰年虫蛋白质分类提取和工艺优化及抗氧化性

Extraction Optimization and Antioxidant Activity of Water-Soluble Protein from Chirocephalus diaphanous

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