响应面法优化发芽大豆富集多肽的培养液组分

doi:10.7506/spkx1002-6630-201124036

食品科学 ›› 2011, Vol. 32 ›› Issue (24): 172-177.doi: 10.7506/spkx1002-6630-201124036

响应面法优化发芽大豆富集多肽的培养液组分

白青云1，唐军涛2，顾振新2

1.淮阴工学院生命科学与化学工程学院 2.南京农业大学食品科技学院

出版日期:2011-12-25 发布日期:2011-12-29
基金资助:
淮阴工学院科研基金面上项目(HGB0909)

Optimization of Culture Solution for Polypeptide Accumulation in Germinated Soybeans by Response Surface Methodology

BAI Qing-yun1，TANG Jun-tao2，GU Zhen-xin2

(1. School of Life Science and Chemical Engineering, Huaiyin Institute of Technology, Huaian 223003, China； 2. College of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, China)

Online:2011-12-25 Published:2011-12-29

摘要/Abstract

摘要： 以浸润方式培养大豆，考察赤霉素(GA3)、CaCl2等10种添加物对发芽大豆多肽富集的影响，对呈显著正效应的添加物进行最适浓度优化，并探讨各组分浓度对发芽大豆多肽富集的影响。结果表明：GA3、CaCl2、AlCl3和MnSO4对发芽大豆多肽富集有显著正效应；Box-Behnken试验结果显示，发芽大豆中多肽含量与培养液组分浓度之间的二次多项模型显著(P＜0.05)，可预测发芽大豆中多肽的富集；其中GA3、CaCl2、AlCl3和MnSO4最适浓度分别是61.00μmol/L、11.20mmol/L、10.13mmol/L和12.17mmol/L，在此条件下培养的发芽大豆中多肽含量达到162.57mg/g，分别是对照和原料大豆的2.10倍和3.97倍，由此表明优化后的培养液组分能显著提高发芽大豆多肽含量。

关键词: 发芽大豆, 多肽, 富集, 培养液组分, 响应面

Abstract: The effects of 10 components in the culture solution on the accumulation of polypeptides after 5 days of soybean germination by means of infiltration were investigated. Using Plackett-Burman experiment design, GA3, CaCl2, aluminium chloride (AlCl3) and manganous sulfate (MnSO4) were identified as main factors that significantly positively affect polypeptide accumulation. The quadratic polynomial regression model for polypeptide content in germinated soybean versus the four variables established based on Box-Behnken experiment design was statistically significant (P＜0.05) and could allow the prediction of polypeptide accumulation. The optimal concentrations of GA3, CaCl2, AlCl3 and MnSO4 in the culture solution were 61.00 μmol/L, and 11.20, 10.13, 12.17 mmol/L, respectively. The polypeptide content in germinated soybean cultured under these conditions was 162.57 mg/g, which was 2.10-fold and 3.97-fold higher than the control and non-germinated soybean, respectively. Therefore, the optimized culture solution can significantly increase polypeptide content in germinated soybean.

Key words: germinated soybean, ploypeptide, accumulation, culture solution composition, response surface methodology

中图分类号:

TS214.2

白青云，唐军涛，顾振新. 响应面法优化发芽大豆富集多肽的培养液组分[J]. 食品科学, 2011, 32(24): 172-177.

BAI Qing-yun，TANG Jun-tao，GU Zhen-xin. Optimization of Culture Solution for Polypeptide Accumulation in Germinated Soybeans by Response Surface Methodology[J]. FOOD SCIENCE, 2011, 32(24): 172-177.

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响应面法优化发芽大豆富集多肽的培养液组分

Optimization of Culture Solution for Polypeptide Accumulation in Germinated Soybeans by Response Surface Methodology

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