猴头菇多糖的纯化工艺

doi:10.7506/spkx1002-6630-201124039

食品科学 ›› 2011, Vol. 32 ›› Issue (24): 186-190.doi: 10.7506/spkx1002-6630-201124039

猴头菇多糖的纯化工艺

肖丽霞1，于洪涛1，胡晓松2，马超3，葛邦国3，吴茂玉3,*

1. 扬州大学食品科学与工程学院 2. 中国农业大学食品科学与营养工程学院 3. 中华全国供销合作总社济南果品研究院

出版日期:2011-12-25 发布日期:2011-12-29

Purification of Polysaccharides from Hericium erinaceus Fruit Bodies

XIAO Li-xia1，YU Hong-tao1，HU Xiao-song2，MA Chao3，GE Bang-guo3，WU Mao-yu3,*

(1. College of Food Science and Engineering, Yangzhou University, Yangzhou 225127, China； 2. College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100087, China； 3. Jinan Fruit Research Institute, All China Federation of Supply & Marketing Co-operatives, Jinan 250014, China)

Online:2011-12-25 Published:2011-12-29

摘要/Abstract

摘要： 对猴头菇子实体多糖进行纯化研究。采用树脂吸附法对猴头菇多糖脱色并利用超滤法进行分级除杂。结果表明：猴头菇多糖适宜采用HD-3树脂进行脱色；静态脱色工艺为pH4.5、每100mL添加树脂量15.0g、脱色2.5h，脱色率和多糖得率分别达到84.9%和83.2%；动态脱色工艺控制流速为5BV/h时，脱色率和多糖得率都较高，平均达83.9%和82.3%；最佳超滤工艺为选择截留分子质量为10kD 和100kD的两种膜对猴头菇多糖进行超滤，超滤温度45℃、压力0.16MPa、溶液pH7～9。此工艺适合工业化生产的需要，得到的猴头菇多糖纯度达74.2%。

关键词: 猴头菇, 多糖, 树脂脱色, 超滤

Abstract: Polysaccharide purification from the fruit bodies of Hericium erinaceus was studied. Crude polysaccharides were extracted from the fruit bodies of Hericium erinaceus by hot water extraction, decolorized by resin adsorption, and ultrafiltrated for the removal of impurities and fractionation. HD-3 resin was found to be the best resin for polysaccharide decoloriztion among 6 ones under investigation. A decolorization rate of 84.9% and a polysaccharide recovery of 83.2% were obtained after 2.5 h of static decolorization at pH 4.5 and a resin dosage of 1.50 g/100 mL of polysaccharide solution. A flow rate of 5 BV/h for dynamic decolorization resulted in higher decolorization rate and polysaccharide recovery, averaging at 83.9% and 82.3%, respectively. The best ultrafiltration results were obtained by using ultrafiltration membranes with molecular weight cutoff (MSCO) of 10 kD and 100 kD respectively and setting the temperature, pressure and sample solution pH as 45 ℃, 0.16 MPa and 7－9, respectively. A polysaccharide purity of 74.2% was achieved after purification under these conditions. The optimized process could meet the requirements of industrial production.

Key words: Hericium erinaceus, polysaccharide, decolorization by resin adsorption, ultrafiltration

中图分类号:

Q539
TS244.2

肖丽霞，于洪涛，胡晓松，马超，葛邦国，吴茂玉. 猴头菇多糖的纯化工艺[J]. 食品科学, 2011, 32(24): 186-190.

XIAO Li-xia，YU Hong-tao，HU Xiao-song，MA Chao，GE Bang-guo，WU Mao-yu. Purification of Polysaccharides from Hericium erinaceus Fruit Bodies[J]. FOOD SCIENCE, 2011, 32(24): 186-190.

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猴头菇多糖的纯化工艺

Purification of Polysaccharides from Hericium erinaceus Fruit Bodies

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