食品科学 ›› 2011, Vol. 32 ›› Issue (24): 191-195.doi: 10.7506/spkx1002-6630-201124040

• 工艺技术 • 上一篇    下一篇

响应曲面法优化野生马兰头总黄酮的提取工艺

夏海涛,刘玉芬,董超男,陈龙龙   

  1. 淮海工学院化学工程学院
  • 出版日期:2011-12-25 发布日期:2011-12-29

Optimization of Extraction Process for Total Flavonoids from Wild Kalimeris indica L. by Response Surface Methodology

XIA Hai-tao,LIU Yu-fen,DONG Chao-nan,CHEN Long-long   

  1. (College of Chemical Engineering, Huaihai Institute of Technology, Lianyungang 222005, China)
  • Online:2011-12-25 Published:2011-12-29

摘要: 以连云港花果山野生马兰头地上部分为原料,研究马兰头黄酮的提取工艺。在单因素试验基础上,根据Box-Behnken试验设计原理,选取提取温度、提取时间、液料比和乙醇体积分数四因素三水平进行中心组合试验,建立黄酮提取率的二次回归方程,确定提取工艺的优化组合条件。结果表明:提取工艺条件为提取温度71℃、提取时间105min、液料比26.5:1(mL/g)、乙醇体积分数63%,黄酮提取率达到最大值。该条件下黄酮提取率预测值为1.65%,验证值为1.62%。

关键词: 马兰头, 总黄酮, 提取, 响应曲面法

Abstract: The extraction of total flavonoids from the aboveground parts of Kalimeris indica L. wildly grown in Huaguoshan Mountain, Lianyungang was optimized. Based on one-factor-at-a-time experiments, a 4-variable, 3-level central composite design was carried out to establish a quadratic regression model for the extraction efficiency of total flavonoids as a function of temperature, time, liquid-to-solid ratio and ethanol concentration. The model was analyzed by response surface methodology to find optimal conditions for the extraction of total flavonoids. The highest extraction efficiency of total flavonoids, of which the predicted and experimental values were 1.65% and 1.62%, respectively, was achieved after 105 min of extraction at 71 ℃ using 63% aqueous ethanol as the extraction solvent at a liquid-to-solid ratio of 26.5:1 (mL/g).

Key words: Kalimeris indica L., total flavonoids, extraction, response surface methodology (RSM)

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